BCIP Stable Liquid Substrate 2.31mM BCIP

BCIP is an excellent substrate for alkaline phosphatase (AP). Upon hydrolysis of the phosphate ester by AP, the resulting halogenated indigo polymerizes to an insoluble blue dimer at the site(s) of enzyme activity.

When reacted with alkaline phosphatase, the free halogenated indolyl derivative dimerizes forming an insoluble blue indigo dye. This product is useful for immunoblotting and immunohistochemical procedures as well as in situ hybridization.
Thus BCIP is an excellent substrate for membrane based assays where a blue endpoint is desired.
MyBioSource provides a stable BCIP solution that has been proven useful for detection of AP probes using a variety of blotting techniques.

Small volumes of BCIP Stable Liquid Substrate 2.31mM BCIP substrate vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS639216 is a substrate targeting BCIP Stable Liquid Substrate 2.31mM BCIP.