Biotin-Linked Polyclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A)

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008]

HSP70: a critical chaperone protein that has a high affinity for unfolded polypeptide chains. It binds extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In cooperation with other chaperones, hsp70 stabilizes preexistent proteins against aggregation and mediates the folding of newly translated polypeptides in the cytosol as well as within organelles. Mitochondrial HSP70 is crucial to the import process: mutant forms of HSP70 fail to import precursor proteins. Is anti-apoptotic in sympathetic neurones and mediates this effect primarily by suppressing c-Jun transcriptional signalling. Interacts with tau protein and mediates proper folding of tau. Can promote the degradation of tau protein. Triptolide, a potential therapeutic agent for progression/metastasis of pancreatic cancer, causes pancreatic cancer cell death by induction of apoptosis, an effect mediated by the inhibition of HSP70.

Protein type: Chaperone; Heat shock protein; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6p21.33

Cellular Component: aggresome; blood microparticle; centriole; centrosome; cytoplasm; cytosol; endoplasmic reticulum; extracellular exosome; extracellular region; ficolin-1-rich granule lumen; focal adhesion; inclusion body; intracellular ribonucleoprotein complex; mitochondrion; nuclear speck; nucleoplasm; nucleus; perinuclear region of cytoplasm; protein complex; ubiquitin ligase complex; vesicle

Molecular Function: ATP binding; ATPase activity; ATPase activity, coupled; cadherin binding; denatured protein binding; enzyme binding; G-protein coupled receptor binding; heat shock protein binding; histone deacetylase binding; protein binding; protein N-terminus binding; receptor binding; RNA binding; ubiquitin protein ligase binding; unfolded protein binding; viral receptor activity

Biological Process: ATP metabolic process; cellular heat acclimation; cellular response to heat; cellular response to oxidative stress; chaperone-mediated protein complex assembly; entry of virus into host cell; mRNA catabolic process; negative regulation of apoptosis; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway; negative regulation of extrinsic apoptotic signaling pathway in absence of ligand; negative regulation of inclusion body assembly; negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway; negative regulation of protein ubiquitination; negative regulation of transforming growth factor beta receptor signaling pathway; neutrophil degranulation; positive regulation of gene expression; positive regulation of interleukin-8 production; positive regulation of microtubule nucleation; positive regulation of mRNA endonucleolytic cleavage involved in unfolded protein response; positive regulation of NF-kappaB transcription factor activity; positive regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein refolding; protein stabilization; regulation of cell death; regulation of cellular response to heat; regulation of mitotic spindle assembly; regulation of mRNA stability; regulation of protein ubiquitination; response to unfolded protein