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> Blocking Buffer For Fluorescent Western Blotting 3-Pack

Blocking Buffer For Fluorescent Western Blotting 3-Pack

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Référence orb348638-3x500ml

Conditionnement : 3x500ml

Marque : Biorbyt

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Blocking Buffer For Fluorescent Western Blotting 3-Pack

SKU: orb348638

Description

Blocking Buffer For Fluorescent Western Blotting 3-Pack

Research Area

Metabolism Research

Images & Validation

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Application Notes
Fluorescence technology is widely used to detect proteins in both the visible and near-infrared ranges. This product allows for superior signal detection and lower background noise when fluorochrome conjugated antibodies are used to visualize proteins in western blotting and other applications. Antibody conjugates prepared with IRDye 800 and IRDye 700DX (Licor), Cy2, Cy3, Cy3.5, Cy5 and Cy5.5 (GE Healthcare), DyLight405, DyLight 549, DyLight 649, DyLight 680, and DyLight 800 (Thermo Fisher/Pierce) and Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 546, Alexa Fluor 647 and Alexa Fluor 680 (Invitrogen/Molecular Probes) have been validated on various platforms using this product with superior results compared to other commercially available products. In the infrared range, where little to no autofluorescence occurs, specific signal is sharply evident from any background giving the best possible signal-to-noise ratio. This allows for detection levels in the picogram range which rivals the sensitivity of chemiluminescence on film for western blotting. Superior results are also seen when this product is used for simultaneous labeling (multiplex) in western blots or microscopy using various fluorochrome combinations for multicolor imaging. Membranes blocked with this product can be dried and are very stable. Membranes that are stored protected from light can be re-washed and/or rescanned.

Key Properties

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PurityBlocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerosal added as an antimicrobial agent.
ConjugationUnconjugated

Storage & Handling

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StorageStore blocking buffer at 4° C prior to opening. DO NOT FREEZE.
Form/AppearanceLiquid (sterile filtered)
Buffer/PreservativesPreservative: Thimerosal is added as an antimicrobial agent.; Buffer: See application note.
Concentration1X
Expiration Date6 months from date of receipt.
Hazard InformationNon-Toxic
DisclaimerFor research use only

Alternative Names

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Multiplex Blocking Buffer, Fluorescent Blocking Buffer, Blocking Solution, Blocking Buffer Western Blot, IRDye Western Blot Blocking Buffer, Alexa Dye Blocking Buffer, DyLight Blocking Buffer

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol