Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.
 
> CAMK2A (Calcium/Calmodulin-dependent Protein Kinase Type II Subunit alpha, CaM-kinase II Subunit alpha, CaM Kinase II Subunit alpha, CAMKA, KIAA0968) (PE)

CAMK2A (Calcium/Calmodulin-dependent Protein Kinase Type II Subunit alpha, CaM-kinase II Subunit alpha, CaM Kinase II Subunit alpha, CAMKA, KIAA0968) (PE)

Imprimer Imprimer
Identifiez-vous

Référence 217244-PE-100ul

Conditionnement : 100ul

Marque : US Biological

Contactez votre distributeur local :


Téléphone : +1 850 650 7790

217244-PECAMK2A (Calcium/Calmodulin-dependent Protein Kinase Type II Subunit alpha, CaM-kinase II Subunit alpha, CaM Kinase II Subunit alpha, CAMKA, KIAA0968) (PE)

Specifications
Clone TypePolyclonal
Hostrabbit
Sourcerat
Swiss ProtP11275
IsotypeIgG
GradeAffinity Purified
ApplicationsWB
CrossreactivityBo Ch Gp Hm Hu Mo Rb Rt
Shipping TempBlue Ice
Storage Temp4°C Do Not Freeze

The Ca2+/Calmodulin-Dependent Kinase family includes CaM Kinases I through IV, Phosphorylase Kinase, and MLCK. The CaM Kinases are inactive in the absence of stimuli; binding to Ca2+/Calmodulin induces conformational changes and activation. CaM Kinases have broad substrate specificity, with many cellular functions.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Control:
HEK293 cell lysate

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Pab|Isotype: IgG|Host: rabbit|Source: rat|Concentration: As Reported |Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).|Purity: Purified by immunoaffinity chromatography.|Immunogen: Synthetic peptide corresponding to the N-terminal of rat CaMK II.|Specificity: Recognizes rat CaMK II. Species Crossreactivity: human, mouse, bovine, hamster, guinea pig, chicken and rabbit.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Synthetic peptide corresponding to the N-terminal of rat CaMK II.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes rat CaMK II. Species Crossreactivity: human, mouse, bovine, hamster, guinea pig, chicken and rabbit.
References
1. Downregulation of delta CaM kinase II in human tumor cells.Tombes, R M, et al. (1999) Biochim. Biophys. Acta, 1452: 1-11 (1999). 2. Regulation of DLG localization at synapses by CaMKII-dependent phosphorylation. Koh, Y H, et al. (1999) Cell, 98: 353-63 (1999). 3. Inhibition of calcium/calmodulin-dependent protein kinase II in rat hippocampus attenuates morphine tolerance and dependence. Fan, G H, et al. (1999) Mol. Pharmacol., 56: 39-45 (1999). 4. Identification and expression of delta-isoforms of the multifunctional Ca2+/calmodulin-dependent protein kinase in failing and nonfailing human myocardium. Hoch, B, et al. (1999) Circ. Res., 84: 713-21 (1999). 5. Regional distribution of type II Ca2+/calmodulin-dependent protein kinase in rat brain. Erondu, N E and Kennedy, M B (1985) J. Neurosci., 5: 3270-7 (1985). 6. AlphaCaMKII binding to the C-terminal tail of NMDA receptor subunit NR2A and its modulation by autophosphorylation. Gardoni, F, et al. (1999) FEBS Lett., 456: 394-8 (1999). 7. CaMKII regulates the density of central glutamatergic synapses in vivo. Rongo, C and Kaplan, J M (1999) Nature, 402: 195-9 (1999).