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> CAMKII alpha/CAMK2A Protein, Human, Recombinant (GST)

CAMKII alpha/CAMK2A Protein, Human, Recombinant (GST)

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Référence TMPY-04373-20ug

Conditionnement : 20ug

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CAMKII alpha/CAMK2A Protein, Human, Recombinant (GST)

(Synonyms: CaMKII α/CAMK2A, CAMKA, calcium/calmodulin-dependent protein kinase II α, calcium/calmodulin-dependent protein kinase II alpha) Copy Product Info
Ca2+/calmodulin-dependent protein kinase2A (CAMK2A) belongs to the serine/threonine protein kinase family and, together with other 28 different isoforms, belongs to the Ca2+/ calmodulin-dependent protein kinase subfamily. CaM kinase Ⅱ is thought to be an important mediator of learning and memory and is also necessary for Ca2+homeostasis and reuptake in cardiomyocytes chloride transport in epithelia, positive T-cell selection, and CD8 T-cell activation. CAMKIIA is one of the major forms of CAMKII. It has been found to play a critical role in sustaining activation of CAMKII at the postsynaptic density. Studies have found that knockout mice without CAMKIIA demonstrate a low frequency of LTP. Additionally, these mice do not form persistent, stable place cells in the hippocampus.
CAMKII alpha/CAMK2A Protein, Human, Recombinant (GST)
For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity
Bioactivity
The specific activity was determined to be 160 nmol/min/mg using Autocamtide-2 synthetic peptide (KKALRRQETVDAL-amide) as substrate.
Description
Ca2+/calmodulin-dependent protein kinase2A (CAMK2A) belongs to the serine/threonine protein kinase family and, together with other 28 different isoforms, belongs to the Ca2+/ calmodulin-dependent protein kinase subfamily. CaM kinase Ⅱ is thought to be an important mediator of learning and memory and is also necessary for Ca2+homeostasis and reuptake in cardiomyocytes chloride transport in epithelia, positive T-cell selection, and CD8 T-cell activation. CAMKIIA is one of the major forms of CAMKII. It has been found to play a critical role in sustaining activation of CAMKII at the postsynaptic density. Studies have found that knockout mice without CAMKIIA demonstrate a low frequency of LTP. Additionally, these mice do not form persistent, stable place cells in the hippocampus.
Species
Human
Expression System
Baculovirus Insect Cells
TagN-GST
Accession NumberQ7LDD5
ConstructionA DNA sequence encoding the human CAMK2A isoform 2 (NP_741960.1) (Met 1-His 478) was fused with the GST tag at the N-terminus. Predicted N terminal: Met
Protein Purity
> 85 % as determined by SDS-PAGE
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationSupplied as sterile 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, 0.5 mM Reduced Glutathione, pH 8.0.
ReconstitutionA Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
SynonymsCaMKII α/CAMK2A, CAMKA, calcium/calmodulin-dependent protein kinase II α, calcium/calmodulin-dependent protein kinase II alpha
Research Background
Ca2+/calmodulin-dependent protein kinase2A (CAMK2A) belongs to the serine/threonine protein kinase family and, together with other 28 different isoforms, belongs to the Ca2+/ calmodulin-dependent protein kinase subfamily. CaM kinase Ⅱ is thought to be an important mediator of learning and memory and is also necessary for Ca2+homeostasis and reuptake in cardiomyocytes chloride transport in epithelia, positive T-cell selection, and CD8 T-cell activation. CAMKIIA is one of the major forms of CAMKII. It has been found to play a critical role in sustaining activation of CAMKII at the postsynaptic density. Studies have found that knockout mice without CAMKIIA demonstrate a low frequency of LTP. Additionally, these mice do not form persistent, stable place cells in the hippocampus.
Chemical Properties
Molecular Weight80.3 kDa (predicted); 80 kDa (reducing conditions)
Storage & Solubility Information
ShippingProteins are shipped with blue ice.
StorageIt is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.