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Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.

Caspase 1 Activity Assay Kit(Colorimetric Method)

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Conditionnement : 100Assays

Téléphone : +1 850 650 7790

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Téléphone : +1 850 650 7790

Caspase 1 Activity Assay Kit(Colorimetric Method) (E-CK-A381)

Manual MSDS

All	Size	Price	Qty
	100Assays	$ 228.00	- +
	20Assays	$ 68.00	- +

For research use only. Order now, ship in 3 days

Detection Principle	Caspase 1, also known as Interkeukin 1β Conberting Enzyme (ICE), is the only caspase in the caspase family that can cleave IL-1β prefixes or IL-18 prefabrication. Caspase 11 can cleave 45 kD caspase 1 preparation to produce 20 kD and 10 kD fragments, which can form heterodimer and further form tetramer by two heterodimers. Caspase 1 regulates apoptosis by cleaving Bcl-XL and the associated immune response by cleaving cytokine precursors, or regulates pyroptosis by hydrolyzing Gastermin D (GSDMD). This caspase 1 activity assay kit is based on caspase 1 that can catalyze the substrate AC-YVAD-pNA to generate yellow pNA (p-Nitroaniline). The pNA has a strong absorption near 405 nm, The activity of caspase 1 can be calculated by determining the absorbance at 405 nm.
Detection Method	Colorimetric method
Sample Type	Cell and tissue samples
Assay Time	4-6 hours
Detection Instrument	Spectrophotometer;Microplate reader
Other Reagents Required	PBS(E-BC-R187), Bradford Protein Determination Kit(E-BC-K168-S or E-BC-K168-M)
Storage	This product can be stored at -20°C for 12 months with shading light.
Expiration date	12 months
Shipping	Ice bag

Cat.No.	Product Name	Sizes
E-CK-A382	Caspase 2 Activity Assay Kit(Colorimetric Method)	100Assays , 20Assays
E-CK-A383	Caspase 3/7 Activity Assay Kit(Colorimetric Method)	100Assays , 20Assays
E-CK-A384	Caspase 4 Activity Assay Kit(Colorimetric Method)	100Assays , 20Assays