Caspase 3 (BSA & Azide Free) (CPP-32, Apoptain, Yama, SCA-1) (PE)
Référence MBS6467484-5x0,1mL
Conditionnement : 5x0.1mL
Marque : MyBiosource
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Western Blot
Caspase 3 (CASP3) Antibody – Rabbit Polyclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6467484MBS6467484 is an antibody targeting CASP3. Also known as Caspase 3 (BSA & Azide Free) (CPP-32, Apoptain, Yama, SCA-1) (PE). Reactive in Human, Bovine, Canine, Hamster, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep. Host: Rabbit. More details
Product Overview
Online Datasheet in PDFProduct Name
Caspase 3 (CASP3), Polyclonal Antibody
Full Product Name
Caspase 3 (BSA & Azide Free) (CPP-32, Apoptain, Yama, SCA-1) (PE)
Product Gene Name
anti-CASP3 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Bovine, Canine, Hamster, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human Caspase 3 (CPP32) at 32kD. Species Crossreactivity: Mouse, rat, monkey, rabbit, hamster, bovine, sheep, porcine and canine.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant full length human caspase 3 protein. Cellular Localization: Predominantly cytoplasmic with some nuclear staining.
Conjugate
PE
Positive Control
Jurkat cells and Tonsil
Note
Preservative Free
Product Categories/Family for anti-CASP3 antibody
Antibodies; Apoptosis; Caspase
Applications Tested/Suitable for anti-CASP3 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-CASP3 antibody
IHC-P: 5-10ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
WB: 2.5-5ug/ml for 2hrs at RT
Applications are based on unconjugated antibody.
WB: 2.5-5ug/ml for 2hrs at RT
Applications are based on unconjugated antibody.
Product References and Citations for anti-CASP3 antibody
1. Krajewska, M., et al., Cancer Res. 57(8): 1605-1613 (1997). 2. Mallat, Z., et al., Circulation 96(2): 424-428 (1997).
Other Notes
Small volumes of anti-CASP3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for CASP3. It may not necessarily be applicable to this product.
Protein Family
Caspase
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.