CB2 Antibody (C-term)
Référence MBS9203911-0,08mL
Conditionnement : 0.08mL
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Immunohistochemical analysis of paraffin-embedded R. brain section using CB2 Antibody(C-term). MBS9203911 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded H. brain section using CB2 Antibody(C-term). MBS9203911 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Flow cytometric analysis of Jurkat cells using Park7 (DJ-1) Antibody(green) compared to an isotype control of rabbit IgG(blue). MBS9203911 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
CB2 Antibody (C-term) western blot analysis in A431 cell line lysates (35ug/lane).This demonstrates the CB2 antibody detected the CB2 protein (arrow).
CB2 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CB2 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
CB2 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CNR2 Antibody – Rabbit Polyclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9203911The cannabinoid delta-9-tetrahydrocannabinol is the principal psychoactive ingredient of marijuana. The proteins encoded by this gene and the cannabinoid receptor 1 (brain) (CNR1) gene have the characteristics of a guanine nucleotide-binding protein… More details
Product Overview
Online Datasheet in PDFProduct Name
CB2 (CNR2), Polyclonal Antibody
Full Product Name
CB2 Antibody (C-term)
Product Gene Name
anti-CNR2 antibody
Product Synonym Names
Cannabinoid receptor 2; CB-2; CB2; hCB2; CX5; CNR2; CB2A; CB2B
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Specificity
This CB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 329-356 amino acids from the C-terminal region of human CB2.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
CB2 peptide (MBS9217774) is used for blocking the activity of CB2 antibody (MBS9203911)
Immunogen Sequence Positions
329-356
OMIM
605051
Cellular Location
Cell membrane; Multi-pass membrane protein. Cell projection, dendrite. Perikaryon Note=Localizes to apical dendrite of pyramidal neurons.
Tissue Location
Preferentially expressed in cells of the immune system with higher expression in B-cells and NK cells (at protein level). Expressed in skin in suprabasal layers and hair follicles (at protein level). Highly expressed in tonsil and to a lower extent in spleen, peripheral blood mononuclear cells, and thymus. PubMed:14657172 could not detect expression in normal brain. Expressed in brain by perivascular microglial cells and dorsal root ganglion sensory neurons (at protein level). Two isoforms are produced by alternative promoter usage and differ only in the 5'UTR: isoform CB2A is observed predominantly in testis with some expression in brain, while isoform CB2B is predominant in spleen and leukocytes
Function
Heterotrimeric G protein-coupled receptor for endocannabinoid 2-arachidonoylglycerol mediating inhibition of adenylate cyclase. May function in inflammatory response, nociceptive transmission and bone homeostasis.
Product Categories/Family for anti-CNR2 antibody
Crown Antibodies; Neuroscience; Loading Control
Applications Tested/Suitable for anti-CNR2 antibody
ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC), Western Blot (WB)
Application Notes for anti-CNR2 antibody
IHC-P~~01:25:00.000
FC~~1:25
WB~~1:1000
E~~ Use at an assay concentration
FC~~1:25
WB~~1:1000
E~~ Use at an assay concentration
Immunohistochemistry (IHC) of anti-CNR2 antibody
Immunohistochemical analysis of paraffin-embedded R. brain section using CB2 Antibody(C-term). MBS9203911 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunohistochemistry (IHC) of anti-CNR2 antibody
Immunohistochemical analysis of paraffin-embedded H. brain section using CB2 Antibody(C-term). MBS9203911 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Flow Cytometry (FC) of anti-CNR2 antibody
Flow cytometric analysis of Jurkat cells using Park7 (DJ-1) Antibody(green) compared to an isotype control of rabbit IgG(blue). MBS9203911 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Immunohistochemistry (IHC) of anti-CNR2 antibody
CB2 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CB2 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product References and Citations for anti-CNR2 antibody
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Larrinaga, G., et al. Histol. Histopathol. 25(9):1133-1138(2010)
Pinheiro, A.P., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (5), 1070-1080 (2010) :
Taylor, A.H., et al. Histochem. Cell Biol. 133(5):557-565(2010)
De Jesus, M.L., et al. Neurochem. Int. 56 (6-7), 829-833 (2010) :
Larrinaga, G., et al. Histol. Histopathol. 25(9):1133-1138(2010)
Pinheiro, A.P., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (5), 1070-1080 (2010) :
Taylor, A.H., et al. Histochem. Cell Biol. 133(5):557-565(2010)
De Jesus, M.L., et al. Neurochem. Int. 56 (6-7), 829-833 (2010) :
Other Notes
Small volumes of anti-CNR2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CNR2 purchase
MBS9203911 is an antibody targeting CNR2. Also known as CB2 Antibody (C-term). Reactive in Human. Host: Rabbit.
Additional Details
NCBI/Uniprot data below describe general gene information for CNR2. It may not necessarily be applicable to this product.
NCBI Official Full Name
cannabinoid receptor 2
NCBI Official Synonym Full Names
cannabinoid receptor 2 (macrophage)
NCBI Official Symbol
CNR2
NCBI Official Synonym Symbols
CB2; CX5; CB-2
UniProt Protein Name
Cannabinoid receptor 2
UniProt Synonym Protein Names
CX5
Protein Family
Cannabinoid receptor
UniProt Gene Name
CNR2
UniProt Synonym Gene Names
CB2A; CB2B; CB-2; CB2; hCB2
UniProt Entry Name
CNR2_HUMAN
NCBI Protein Information
cannabinoid receptor 2
UniProt Comments for CNR2
CNR2: Heterotrimeric G protein-coupled receptor for endocannabinoid 2-arachidonoylglycerol mediating inhibition of adenylate cyclase. May function in inflammatory response, nociceptive transmission and bone homeostasis. Belongs to the G-protein coupled receptor 1 family.
Protein type: Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1p36.11
Cellular Component: extrinsic to internal side of plasma membrane; integral to plasma membrane; dendrite; plasma membrane; perikaryon
Molecular Function: cannabinoid receptor activity
Biological Process: negative regulation of mast cell activation; G-protein signaling, coupled to cyclic nucleotide second messenger; negative regulation of nitric-oxide synthase activity; negative regulation of inflammatory response; response to amphetamine; response to lipopolysaccharide; immune response; negative regulation of action potential; sensory perception of pain; inflammatory response; negative regulation of synaptic transmission, GABAergic
Show more Protein type: Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1p36.11
Cellular Component: extrinsic to internal side of plasma membrane; integral to plasma membrane; dendrite; plasma membrane; perikaryon
Molecular Function: cannabinoid receptor activity
Biological Process: negative regulation of mast cell activation; G-protein signaling, coupled to cyclic nucleotide second messenger; negative regulation of nitric-oxide synthase activity; negative regulation of inflammatory response; response to amphetamine; response to lipopolysaccharide; immune response; negative regulation of action potential; sensory perception of pain; inflammatory response; negative regulation of synaptic transmission, GABAergic
Research Articles on CNR2
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with anti-CNR2 antibody
| Reference Product | PubMed Publications |
|---|---|
| GPR55 antibody | >6 publications with CNR2 and GPR55 |
| OPRD1 antibody | >1 publications with CNR2 and OPRD1 |
| GPR18 antibody | >1 publications with CNR2 and GPR18 |
| OPRM1 antibody | >1 publications with CNR2 and OPRM1 |
Pathways associated with anti-CNR2 antibody
| Products by Pathway | Pathway Diagram |
|---|---|
| Thiamine Metabolism Pathway antibodies | Thiamine Metabolism Pathway Diagram |
| Thiamine Metabolism Pathway antibodies | Thiamine Metabolism Pathway Diagram |
| Thiamine Metabolism Pathway antibodies | Thiamine Metabolism Pathway Diagram |
| Thiamine Metabolism Pathway antibodies | Thiamine Metabolism Pathway Diagram |
| Thiamine Metabolism Pathway antibodies | Thiamine Metabolism Pathway Diagram |
| Thiamine Metabolism Pathway antibodies | Thiamine Metabolism Pathway Diagram |
| Thiamine Metabolism Pathway antibodies | Thiamine Metabolism Pathway Diagram |
| Thiamine Metabolism Pathway antibodies | Thiamine Metabolism Pathway Diagram |
| Thiamine Metabolism Pathway antibodies | Thiamine Metabolism Pathway Diagram |
| Thiamine Metabolism Pathway antibodies | Thiamine Metabolism Pathway Diagram |
Diseases associated with anti-CNR2 antibody
| Disease Name | Pubmed Publications |
|---|---|
| Pain Antibodies | >48 publications with CNR2 and Pain |
| Inflammation Antibodies | >46 publications with CNR2 and Inflammation |
| Disease Models, Animal Antibodies | >40 publications with CNR2 and Disease Models, Animal |
| Necrosis Antibodies | >18 publications with CNR2 and Necrosis |
| Hyperalgesia Antibodies | >17 publications with CNR2 and Hyperalgesia |
| Immune System Diseases Antibodies | >17 publications with CNR2 and Immune System Diseases |
| Heart Diseases Antibodies | >12 publications with CNR2 and Heart Diseases |
| Edema Antibodies | >11 publications with CNR2 and Edema |
| Fibrosis Antibodies | >8 publications with CNR2 and Fibrosis |
| Fatty Liver Antibodies | >6 publications with CNR2 and Fatty Liver |
Organs/Tissues associated with anti-CNR2 antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Blood Antibodies | >44 publications with CNR2 and Blood |
| Liver Antibodies | >24 publications with CNR2 and Liver |
| Bone Marrow Antibodies | >7 publications with CNR2 and Bone Marrow |
| Placenta Antibodies | >2 publications with CNR2 and Placenta |
| Uncharacterized Tissue Antibodies | >1 publications with CNR2 and Uncharacterized Tissue |