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> CD28 (Biotin)

CD28 (Biotin)

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Référence 214642-Biotin-100ul

Conditionnement : 100ul

Marque : US Biological

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214642-BiotinCD28 (Biotin)

Specifications
Clone TypeMonoclonal
Hostmouse
Sourcebovine
IsotypeIgG1
GradeAffinity Purified
ApplicationsFC
CrossreactivityBo
Shipping TempBlue Ice
Storage Temp-20°C

The bovine homologue of human CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2).

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:25-1:200
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: CC219|Host: mouse|Source: bovine|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.|Purity: Purified by Protein A affinity chromatography.|Immunogen: Plasmid DNA encoding the bovine CD28 gene|Specificity: Recognizes the bovine homologue of human CD28||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Plasmid DNA encoding the bovine CD28 gene
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes the bovine homologue of human CD28
References
1. Hogg A.E. et al (2011) Characterization of age-related changes in bovine CD8+ T-cells Vet. Imm. & Immunopath. 140:47–54