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> CD45 (LCA) (Biotin)

CD45 (LCA) (Biotin)

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Référence 214696-Biotin-100ul

Conditionnement : 100ul

Marque : US Biological

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214696-Biotin CD45 (LCA) (Biotin)

Clone Type
Monoclonal
Host
mouse
Source
sheep
Isotype
IgG1
Grade
Affinity Purified
Applications
FC IHC IP
Crossreactivity
Bo Gt Sh
Shipping Temp
Blue Ice
Storage Temp
-20°C

CD45 (Leukocyte common antigen), which is expressed on all ovine lymphocytes, macrophages and granulocytes. Immunoprecipitates CD45 molecules of 190kD, 210kD and 225kD from lymph node lysates.

Applications:
Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: Frozen and paraffin sections
Optimal dilutions to be determined by the researcher.

Hybridoma:
NS1 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 1.11.32|Host: mouse|Source: sheep|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.|Purity: Purified by Protein G affinity chromatography from tissue culture supernatant.|Immunogen: Ovine efferent lymphatic duct lymphocytes.|Specificity: Recognizes sheep CD45. Species Crossreactivity: bovine and goat ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Ovine efferent lymphatic duct lymphocytes.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes sheep CD45. Species Crossreactivity: bovine and goat
References
1. Mackay, C. et al. (1987) A monoclonal antibody to the p220 component of sheep LCA identifies B cells and a unique lymphocyte subset. Cell. Immunol. 110: 46-55. 2. Zannettino, A.C. et al. (2010) Comparative Assessment of the Osteoconductive Properties of Different Biomaterials In Vivo Seeded With Human or Ovine Mesenchymal Stem/Stromal Cells. Tissue Eng Part A. Jul 28. [Epub ahead of print] 3. Mackay, C. et al. (1989) Gamma/delta T cells express a unique surface molecule appearing late during thymic development. Eur. J. Immunol. 19: 1477-1483. 4. Maddox, J. et al. (1985) The sheep analogue of leucocyte common antigen (LCA). Immunology. 55: 347-353. 5. Breugelmans, S. et al. (2010) Immunoassay of lymphocyte subsets in ovine palatine tonsils. Acta Histochem. May 21. [Epub ahead of print] 6. Breugelmans, S. et al. (2011) Differences between the ovine tonsils based on an immunohistochemical quantification of the lymphocyte subpopulations. Comp Immunol Microbiol Infect Dis. 34: 217-25. 7. Herdrich, B.J. et al. (2010) Regenerative healing following foetal myocardial infarction. Eur J Cardiothorac Surg. 38: 691-8. 8. Reichert, J.C. et al. (2010) Ovine bone- and marrow-derived progenitor cells and their potential for scaffold-based bone tissue engineering applications in vitro and in vivo. J Tissue Eng Regen Med. 4: 565-76. 9. Galinsky, R. et al. (2011) Effect of intra-amniotic lipopolysaccharide on nephron number in preterm fetal sheep. Am J Physiol Renal Physiol. May 18. [Epub ahead of print] 10. Kallapur, S.G. et al. (2011) Pulmonary and Systemic Inflammatory Responses to Intraamniotic IL-1 alpha in fetal sheep. Am J Physiol Lung Cell Mol Physiol. Jun 10. [Epub ahead of print] 11. Stickler, P, et al (2010) Cyclically stretching developing tissue in vivo enhance mechanical strength and organization of vascular graphs Acta Biomaterialia 6: 2448-2456 12. Berardinelli, P. et al. (2012) Role of amniotic fluid mesenchymal cells engineered on MgHA/collagen-based scaffold allotransplanted on an experimental animal study of sinus augmentation. Clin Oral Investig. Oct 14. [Epub ahead of print] 13. Geherin, S.A. et al. (2012) The skin, a novel niche for recirculating B cells. J Immunol. 188: 6027-35.