CD56 / NCAM1 (Neuronal Cell Marker) Mouse Monoclonal Antibody

Référence MBS438122-0,1mg-BSA-AZ

Conditionnement : 0.1mg(WithBSA&Azideat0.2mg/mL)

Marque : MyBiosource

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CD56 / NCAM1 (NCAM1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438122

MBS438122 is an antibody targeting NCAM1. Also known as CD56 / NCAM1 (Neuronal Cell Marker) Mouse Monoclonal Antibody. Reactive in Human, Rat, Zebrafish. Host: Mouse. More details

Product Overview

Product Name
CD56 / NCAM1, Monoclonal Antibody
Full Product Name
CD56 / NCAM1 (Neuronal Cell Marker) Mouse Monoclonal Antibody
Product Gene Name
anti-NCAM1 antibody
Product Synonym Names
NCAM, Leu-19, NKH1, MSK39, NCAM120, NCAM140, NCAM180, Neural Cell Adhesion Molecule
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
SPM128
Host
Mouse
Species Reactivity
Human, Rat, Zebrafish
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
This MAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the nodal and nasal NK/T-cell lymphomas.
Preparation and Storage
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
180; 145; 125kDa
OMIM
116930
3D Structure
ModBase 3D Structure for P13591
Cellular Localization
Cell Surface
Immunogen
Membrane preparation of a small cell lung carcinoma
Hu-Chromosome Location
11q23.1
Positive Control
Cerebellum, Pancreas, Neuroblastoma
Applications Tested/Suitable for anti-NCAM1 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes for anti-NCAM1 antibody
Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Optimal dilution for a specific application should be determined.

Immunohistochemistry (IHC) of anti-NCAM1 antibody

Formalin-fixed, paraffin-embedded human Pancreas stained with CD56 Monoclonal Antibody (SPM128)
Mouse Anti-NCAM1 Monoclonal Antibody – Cat# MBS438122

Flow Cytometry (FC/FACS of anti-NCAM1 antibody

FACS analysis of CD56 on human Monocytes using CD56 Monoclonal Antibody (SPM128)
Mouse Anti-NCAM1 Monoclonal Antibody, Flow Cytometry (FC/FACS – Cat# MBS438122

Product References and Citations for anti-NCAM1 antibody

Hilkens et al., Int J Cancer. 1984 34:197-206. Kibbelaar et al. Eur. J. Cancer 1991 27:431-435

Other Notes

Small volumes of anti-NCAM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NCAM1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
neural cell adhesion molecule 1 isoform 1
NCBI Official Synonym Full Names
neural cell adhesion molecule 1
NCBI Official Symbol
NCAM1
NCBI Official Synonym Symbols
CD56; NCAM; MSK39
UniProt Protein Name
Neural cell adhesion molecule 1
UniProt Synonym Protein Names
CD_antigen: CD56
Protein Family
Neural cell adhesion molecule
UniProt Gene Name
NCAM1
UniProt Synonym Gene Names
NCAM; N-CAM-1; NCAM-1
UniProt Entry Name
NCAM1_HUMAN
NCBI Protein Information
neural cell adhesion molecule 1

NCBI Summary for NCAM1

This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]
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UniProt Comments for NCAM1

NCAM1: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Membrane protein, GPI anchor; Cell adhesion

Chromosomal Location of Human Ortholog: 11q23.1

Cellular Component: cell surface; cytoplasm; external side of plasma membrane; Golgi membrane; membrane; plasma membrane

Molecular Function: Ras guanyl-nucleotide exchange factor activity

Biological Process: axon guidance; MAPKKK cascade
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NCAM1 antibody

 Reference Product  PubMed Publications
 ICAM1 antibody  >130 publications with NCAM1 and ICAM1
 ST8SIA2 antibody  >80 publications with NCAM1 and ST8SIA2
 FYN antibody  >51 publications with NCAM1 and FYN
 PTK2 antibody  >24 publications with NCAM1 and PTK2
 ST8SIA4 antibody  >20 publications with NCAM1 and ST8SIA4
 IL2 antibody  >18 publications with NCAM1 and IL2
 SPTBN2 antibody  >1 publications with NCAM1 and SPTBN2
 SPTBN1 antibody  >1 publications with NCAM1 and SPTBN1
 PRNP antibody  >1 publications with NCAM1 and PRNP

Pathways associated with anti-NCAM1 antibody

 Products by Pathway  Pathway Diagram
 ARMS-mediated Activation Pathway antibodies  ARMS-mediated Activation Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Cardiac Progenitor Differentiation Pathway antibodies  Cardiac Progenitor Differentiation Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram

Diseases associated with anti-NCAM1 antibody

 Disease Name  Pubmed Publications
 Brain Diseases Antibodies  >586 publications with NCAM1 and Brain Diseases
 Disease Models, Animal Antibodies  >283 publications with NCAM1 and Disease Models, Animal
 Necrosis Antibodies  >283 publications with NCAM1 and Necrosis
 Inflammation Antibodies  >260 publications with NCAM1 and Inflammation
 Lung Neoplasms Antibodies  >233 publications with NCAM1 and Lung Neoplasms
 Congenital Abnormalities Antibodies  >205 publications with NCAM1 and Congenital Abnormalities
 Liver Diseases Antibodies  >194 publications with NCAM1 and Liver Diseases
 Neoplasm Invasiveness Antibodies  >153 publications with NCAM1 and Neoplasm Invasiveness
 Neurobehavioral Manifestations Antibodies  >120 publications with NCAM1 and Neurobehavioral Manifestations
 Liver Neoplasms Antibodies  >119 publications with NCAM1 and Liver Neoplasms

Organs/Tissues associated with anti-NCAM1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >2875 publications with NCAM1 and Brain
 Blood Antibodies  >2624 publications with NCAM1 and Blood
 Nerve Antibodies  >1822 publications with NCAM1 and Nerve
 Embryonic Tissue Antibodies  >857 publications with NCAM1 and Embryonic Tissue
 Muscle Antibodies  >584 publications with NCAM1 and Muscle
 Bone Antibodies  >550 publications with NCAM1 and Bone
 Skin Antibodies  >392 publications with NCAM1 and Skin
 Vascular Antibodies  >384 publications with NCAM1 and Vascular
 Eye Antibodies  >349 publications with NCAM1 and Eye
 Lung Antibodies  >343 publications with NCAM1 and Lung

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