Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.
 
> CD90 (Thy1.1) (APC)

CD90 (Thy1.1) (APC)

Imprimer Imprimer
Identifiez-vous

Référence C2441-04A-APC-100ul

Conditionnement : 100ul

Marque : US Biological

Contactez votre distributeur local :


Téléphone : +1 850 650 7790

C2441-04A-APCCD90 (Thy1.1) (APC)

Specifications
Clone TypeMonoclonal
Hostmouse
Sourcerat
IsotypeIgG1
GradeAffinity Purified
ApplicationsFC IF IHC IP
CrossreactivityGp Mo Rb Rt
Shipping TempBlue Ice
Storage Temp4°C Do Not Freeze

The Thy-1 antigen is found on a variety of cell types including thymocytes, neuronal cells, T-Lymphocytes (mouse), stem cells and immature B cells (rat) and connective tissue.

Applications:
Suitable for use in Flow Cytometry, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Frozen sections
Optimal dilution determined by the researcher.

Hybridoma:
NS1 myeloma cells with spleen cells from Balb/c mice.

Recommended Negative Control:
I1904-78Y: IgG1, Murine Negative Control (For rat tissue)

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 6A341 (OX-7)|Host: mouse|Source: rat|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).|Purity: Purified by Protein G affinity chromatography from tissue culture supernatant.|Immunogen: Rat Thy1 antigen. Swiss/UniProt Accession: P01830|Specificity: Recognizes rat CD90 also known as Thy1.1. Thy1.1 is a monomorphic determinant in rat but polymorphic in mice. Reacts with Thy1.1 mice e.g. AKR and FVB mice, but not Thy1.2 mice such as CBA and BALB/c. Species Crossreactivity: mouse, guinea pig and rabbit||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Rat Thy1 antigen. Swiss/UniProt Accession: P01830
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes rat CD90 also known as Thy1.1. Thy1.1 is a monomorphic determinant in rat but polymorphic in mice. Reacts with Thy1.1 mice e.g. AKR and FVB mice, but not Thy1.2 mice such as CBA and BALB/c. Species Crossreactivity: mouse, guinea pig and rabbit
References
1. Mason, D.W. et al. (1980) Biochem.J. 187: 1-20. 2. Campbell, D.G. et al. (1981) Biochem. J. 195: 15-30. 3. Bukovský, A. et al. (1983) Immunology. 48:587-595. 4. Lee, W. et al. (1998) Circ. Res. 82: 845-851. 5. Jeng, C .J. et al. (1998) J. Cell. Biol. 140(3): 685-98. 6. Banerjee, S.A. et al. (1997) J. Neurosci. 17: 2756-2765.7. Kawachi, H. et al. (1992) Clin. Exp. Immunol. 88: 399-404.8. Tamura, M. et al. (1996) Biochem Biophys Res Comm.9. Stevenson, K.S. et al. (2009) Stem Cells Dev. 18:1389-98. 10. Biermann, J. et al. (2011) Mol Vis. 17: 395-403. 11. Keller, R.K. et al. (2004) J Lipid Res. 45: 347-5512. Ohashi, N. et al. (2010) Hypertens Res. 33:1174-81.