Inset: IHC and IF of Collagen Type IV on a FFPE Skin Tissue
Collagen Type IV Antibody – Mouse Monoclonal
For Research Use Only. Not for use in diagnostic procedures.Catalog #: MBS370047
Collagen is the main protein of connective tissue in animals and the most abundant protein in mammals, making up about 25% of the total protein content. Collagen IV is a major constituent of the basement membranes, along with laminins and enactins. It is… More details
Product Overview
Online Datasheet in PDF
Product Name
Collagen Type IV, Monoclonal Antibody
Full Product Name
Collagen Type IV
Clonality
Monoclonal
Isotype
IgG1/K
Clone Number
CIV22
Host
Mouse
Species Reactivity
Human, Rat, Canine, Horse
Form/Format
Anti-Collagen Type IV is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Preparation and Storage
Store at 2 to 8 degree C in the dark.
Control
Muscle, Lung
Localization
Cytoplasmic
Reactivity Note
Paraffin, Frozen
Precautions
1. This product contains <0.1% sodium azide (NaN3) as a preservative. Ensure proper handling procedures are used with this reagent. 2. Always wear personal protective equipment such as a laboratory coat, goggles, and gloves when handling reagents. 3. Dispose of unused solution with copious amounts of water. 4. Do not ingest reagent. If reagent is ingested, seek medical advice immediately. 5. Avoid contact with eyes. If contact occurs, flush with large quantities of water. 6. Follow safety precautions of the heating device used for epitope retrieval (TintoRetriever Pressure Cooker or similar). 7. For additional safety information refer to the Safety Data Sheet for thisproduct. 8. For complete recommendations for handling biological specimens, please referto the CDC document,
Mounting Protocol IHC
For detailed instructions using biodegradable permanent mounting media such as XyGreen PermaMounter or organic solvent-based resin such as PermaMounter.
Mounting Protocol IF
1. Bring FluoroMounter or FluoroMounter with DAPI to room temperature. 2. Rinse slides with distilled or deionized water. 3. Remove excess water from slides before laying them flat in the dark. 4. Turn the media bottle upside down before opening the dropper bottle. 5. Apply 1-3 drops of FluoroMounter to each slide making sure the specimen is covered. 6. Incubate 3-5 minutes at room temperature in the dark. 7. Coverslip. 8. Observe under a fluorescent microscope using the appropriate filters. 9. The slides are recommended to be stored at 2-8 0C in the dark.
Product Limitations
Due to inherent variability present in immunohistochemical procedures (including fixation time of tissues, dilution factor of antibody, retrieval method utilized, and incubation time), optimal performance should be established through the use of positive and negative controls.
Preparation for Frozen Tissues Procedure
1. Embed the specimen in OCT inside the cryostat. 2. Cut sections at 5 microns. 3. Place the section on a positively charged glass slide. 4. Air dry for 30-60 minutes. 5. Fix in acetone 100% for 2-10 minutes. 6. Air dry for another 10 minutes.
ISO Certification
Manufactured in an ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-Collagen Type IV antibody
Breast Cancer, Gall Bladder & Pancreatic Cancer
Application Notes for anti-Collagen Type IV antibody
Paraffin sections: The antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. Ensure tissue undergoes appropriate fixation for best results. Pre-treatment of tissues with heat-induced epitope retrieval (HIER) is recommended using Bio SB ImmunoDNA Retriever with Citrate, ImmunoDNA Retriever with EDTA, and/or ImmunoDNA Digestor. See reverse side for complete protocol. Tissue should remain hydrated via use of Immuno/DNA Washer solutions.
Frozen sections and cell preparations: The antibody can be used on acetone-fixed frozen sections and acetone-fixed cell preparations.
Preparation for FFPE Tissues Procedure: 1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positively charged slides such as Hydrophilic Plus Slides. 2. Air dry for 2 hours at 58° C. 3. Deparaffinize, dehydrate, and rehydrate tissues. 4. One of the Epitope Retrieval Methods can be used: 4a. Pre-warm the ImmunoDNA Digestor solution to room temperature. Incubate tissue sections with the ImmunoDNA Digestor solution for 10 minutes at room temperature or for 5 minutes at 37 oC. 4b. Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA and place on the trivet in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 10 minutes. Open and immediately transfer slides to room temperature. Let it cool down for 10 min. Incubate in enzymatic pretreatment with ImmunoDNA Digestor or similar Proteinase K enzyme for 1-2 minutes.
IHC/IF protocol.||For manual IF\IHC, perform antibody incubation at ambient temperature. For automated IF\IHC methods, perform antibody incubation according to instrument manufacturer’s instructions. Wash slides with ImmunoDNA washer or DI water. Continue IF\IHC protocol. Wash slides between each step with ImmunoDNA washer solution.
Dilution Information of anti-Collagen Type IV antibody
Immunohistochemistry (IHC) of anti-Collagen Type IV antibody
Immunofluorescence / Immunohistochemistry (IF/IHC) of anti-Collagen Type IV antibody
Inset: IHC and IF of Collagen Type IV on a FFPE Skin Tissue
Testing Data (TD) of anti-Collagen Type IV antibody
Testing Data (TD) of anti-Collagen Type IV antibody
Related Product Information for anti-Collagen Type IV antibody
Collagen is the main protein of connective tissue in animals and the most abundant protein in mammals, making up about 25% of the total protein content. Collagen IV is a major constituent of the basement membranes, along with laminins and enactins. It is composed of the alpha 1 IV chain and alpha 2 IV chain in a 2:1 ratio. It can form insoluble fibers with high tensile strength. Normal tissue stains with this antibody in a manner consistent with the sites of mesenchymal elements and epithelial basal laminae. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas. Collagen IV can also be useful in the classification of soft tissue tumors; Schwanomas, Leiomyomas, and their well-differentiated malignant counterparts usually immunoreact to this antibody. The vascular nature of neoplasms, Hemangiopericytoma, Angiosarcoma and Epithelioid Hemangioendothelioma can be observed with this antibody.
Other Notes
Small volumes of anti-Collagen Type IV antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for Collagen Type IV purchase
MBS370047 is an antibody targeting Collagen Type IV. Reactive in Human, Rat, Canine, Horse. Host: Mouse.
Additional Details
NCBI/Uniprot data below describe general gene information for Collagen Type IV. It may not necessarily be applicable to this product.
Protein Family
Collagen type IV alpha-3-binding protein
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
