CRCP ELISA Kit (Rat)
Référence OKEH06001
Conditionnement : 96T
Marque : Aviva Systems Biology
Contactez votre distributeur local :
Téléphone : +1 850 650 7790
CRCP ELISA Kit (Rat) (OKEH06001)
| Datasheets/Manuals | Printable datasheet for CRCP ELISA Kit (Rat) (OKEH06001) |
|---|
| Predicted Species Reactivity | Rat|Rattus norvegicus | ||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Crcp ELISA Kit (Rat) (OKEH06001) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Crcp has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Crcp is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Crcp captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into Serum and Plasma = 101% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision. Mean Intra-Assay Precision - <4.5%, n = 20 Mean Inter-Assay Precision - <7.5%, n = 20 | ||||||||||||||||||||||
| ELISA Kit Component |
| ||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 0.08 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Rat DNA-directed RNA polymerase III subunit RPC9 | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | Crcp |
|---|---|
| Gene Full Name | CGRP receptor component |
| Alias Symbols | calcitonin gene-related peptide-receptor component protein, Cgrp-rcp, CGRP-receptor component protein, CGRPRCP, DNA-directed RNA polymerase III subunit RPC9, RNA polymerase III subunit C9. |
| NCBI Gene Id | 114205 |
| Protein Name | DNA-directed RNA polymerase III subunit RPC9 |
| Description of Target | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity). |
| Uniprot ID | Q8VHM6 |
| Protein Accession # | NP_446122.1 |
| Nucleotide Accession # | NM_053670.3 |
| Protein Size (# AA) | 148 |
