Creatinine Serum Detection Kit

Colorimetric measurement of creatinine
Creatinine (2-amino-1-methyl-5H-imadazol-4-one) is a metabolite of phosphocreatine (p-creatine), a molecule used as a store for high-energy phosphate that can be utilized by tissues for the production of ATP (1). Creatine either comes from the diet or synthesized from the amino acids arginine, glycine, and methionine. This occurs in the kidneys and liver, although other organ systems may be involved and species-specific differences may exist (2). Creatine and p-creatine are converted non-enzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. In vivo , this conversion appears to be irreversible and in vitro it is favored by higher temperatures and lower pH2. Creatinine forms spontaneously from p-creatine (3). Under normal conditions, its formation occurs at a rate that is relatively constant and as intra-individual variation is <15% from day to day, creatinine is a useful tool for normalizing the levels of other molecules found in urine. Additionally altered creatinine levels may be associated with other conditions that result in decreased renal blood flow such as diabetes and cardiovascular disease (4-6).

1. Wallimann, T. et al., Biochem. J., 2000, 281, 21-40.2. Wyss, M. and Kaddurah-Daouk, R., Physiol. Rev., 2000, 80, 1107-1213.3. Raja Iyengar, M. et al., J. Biol. Chem, 1985, 260, 7562-7567.4. Manjunath, G. et al., Postgrad. Med. 2001, 110, 55-62.5. Gross, J.L. et al., Diabetes Care, 2005, 28, 164-176.6. Anavekar, N.S. et al., New Engl. J. Med., 2004, 351, 1285-1295.

Small volumes of Creatinine Serum kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS8006046 is a kit targeting Creatinine Serum. Also known as Creatinine Serum Detection Kit. Reactive in Human, Mouse, Rabbit, Rat, Sheep.