Dengue IgM ELISA

Intended Uses: The Dengue virus IgG ELISA Kit is intended for the detection of IgG antibody to Dengue virus in human serum or plasma. For Research Use Only

Principle of the Assay: Diluted serum and biotinylated anti-human IgG antibody reagent are added to the wells coated with Streptavidin. IgG antibodies in the serum binds to the x-h IgG antibody, and the complex is immobilized on the plate through streptavidin-biotin interactions. Unbound proteins are washed off through a washing step. Dengue antigen-HRP conjugate reagent is added into the wells. Dengue virus IgG specific antibody, if present, binds to the antigen. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the oxidation of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgG specific antibody in the sample.

Small volumes of Dengue IgM elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS494314 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Dengue IgM, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Dengue IgM. The ELISA analytical biochemical technique of the MBS494314 kit is based on Dengue IgM antibody-Dengue IgM antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Dengue IgM antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Dengue IgM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).