DNase I (RNase & Protease free) [9003-98-9]

DNase I is an enzyme that degrades DNA. DNase I is mainly produced by digestive system organs, such as the pancreas and parotid gland. Three types of DNase I are known in mammals: pancreatic type, parotid type, and pancreatico-parotid type. DNase I plays a key role in the cleavage of extracellular DNA, and is critical for limiting inflammatory responses and maintaining homeostasis. DNase I is responsible for digesting extracellular nucleoproteins, which may be essential for preventing autoimmune responses. Decreased DNase I activity may be associated with the onset and progression of systemic lupus erythematosus (SLE). DNase I (RNase & Protease free) is a molecular biology-grade DNase I purified by chromatography to remove RNase and protease^[1].

As a member of the DNase I family, DNase I cleaves DNA to generate two products with 5′-phosphate and 3′-hydroxyl termini, respectively^[1].
DNase I cleaves double-stranded DNA (dsDNA) 100-500 times more efficiently than single-stranded DNA (ssDNA), while the structure and sequence of DNA substrates affect hydrolysis kinetics. Compared to the relatively hydrolysis-resistant Z-DNA, B-DNA is the most suitable substrate for DNase I^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

• [1]. Lauková L, et al. Deoxyribonucleases and Their Applications in Biomedicine. Biomolecules. 2020;10(7):1036. Published 2020 Jul 11.  [Content Brief]