dNTP Mix dUTP - Solution
Référence NU-1020S
Conditionnement : 1ml
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Premix of 10 mM dATP, dCTP, dGTP and 20 mM dUTP
2'-Deoxyadenosine-5'-triphosphate, Sodium salt; 2'-Deoxycytidine-5'-triphosphate, Sodium salt; 2'-Deoxyguanosine-5'-triphosphate, Sodium salt; 2'-Deoxyuridine-5'-triphosphate, Sodium salt
For general laboratory use.
Please centrifuge briefly before opening (volume ≤2 ml).
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Shelf Life: 12 months
Molecular Formula:
dATP: C10H16N5O12P3 (free acid)
dCTP: C9H16N3O13P3 (free acid)
dGTP: C10H16N5O13P3 (free acid)
dUTP: C9H15N2O14P3 (free acid)
Molecular Weight:
dATP: 491.18 g/mol (free acid)
dCTP: 467.15 g/mol (free acid)
dGTP: 507.18 g/mol (free acid)
dUTP: 468.14 g/mol (free acid)
Purity: ≥ 99 % (HPLC)
Form: clear aqueous solution
pH: 8.5 ±0.2 (22 °C)
Description:
dNTP Mix incl. dUTP is an mixture of 10 mM ultrapure dATP, dCTP, and dGTP, and 20 mM dUTP supplied as clear aqueous solution (pH 8.5). dUTP can be used in place of dTTP in PCR and RT-PCR protocols to prevent carry-over contaminations from previous amplifications.
BIOZ Product Citations:
Powered by Bioz See more details on BiozSelected References:
Erlich et al. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 29 (239):487.
Holland et al. (1991) Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA 88 (16):7276.
Sanger et al. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463.
