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DOTAP chloride [132172-61-3]

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Référence NB-64-02329-25mg

Conditionnement : 25mg

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DOTAP chloride

(Synonyms: 1,2-Dioleoyl-3-trimethylammonium-propane chloride) Copy Product Info

Synonyms: 1,2-Dioleoyl-3-trimethylammonium-propane chloride

Catalog No. T11086 Copy Product Info

Purity: 99.97%

DOTAP chloride (1, 2-dioleoyl-3-trimethylammonium-propane chloride) is an efficient cationic lipid, and its target is mainly the negatively charged components on the cell membrane. DOTAP chloride binds to negatively charged nucleic acids (such as DNA and RNA) by electrostatic interaction to form complexes, thereby achieving the delivery of nucleic acids.

Cas No. 132172-61-3

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.97%

Product Introduction

DOTAP chloride AI Summary

DOTAP chloride exhibits varying levels of toxicity in different assays related to liver function. When complexed with specific compounds such as cholesterol or DOPE, it shows toxic effects on alanine aminotransferase (ALAT) and aspartate aminotransferase (ASAT) levels in mice, with toxicity levels ranging from 37.0 to 657.0. This indicates a significant impact on liver enzymes, suggesting a potential role in disrupting normal liver function and metabolism..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	DOTAP chloride (1, 2-dioleoyl-3-trimethylammonium-propane chloride) is an efficient cationic lipid, and its target is mainly the negatively charged components on the cell membrane. DOTAP chloride binds to negatively charged nucleic acids (such as DNA and RNA) by electrostatic interaction to form complexes, thereby achieving the delivery of nucleic acids.
Targets & IC50	293T cells:58.6 µM
In vitro	METHODS: Prostate cancer cell line PC3 and melanoma cell line M21 were treated with paclitaxel (PTX) loaded on DOTAP/DOPC liposome carriers (CLs) for 24 hours. The cytotoxicity was detected by measuring the absorbance at 490 nm using an enzyme-linked immunosorbent assay reader. RESULTS: With the increase of PTX content, the cytotoxic effect of DOTAP/DOPC CLs is enhanced. [1]
Synonyms	1,2-Dioleoyl-3-trimethylammonium-propane chloride

Chemical Properties

Molecular Weight	698.542
Formula	C₄₂H₈₀ClNO₄
Cas No.	132172-61-3
Smiles	CCCCCCCC/C=C\CCCCCCCC(OCC(OC(CCCCCCC/C=C\CCCCCCCC)=O)C[N+](C)(C)C)=O.[Cl-]
Relative Density.	no data available

Storage & Solubility Information

Storage

Store at low temperature Pure form: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

Ethanol: 130.00 mg/mL (186.10 mM), Sonication and heating are recommended.

DMSO: 125.00 mg/mL (178.94 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 4.00 mg/mL (5.73 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO/Ethanol

	1mg	5mg	10mg	50mg
1 mM	1.4316 mL	7.1578 mL	14.3155 mL	71.5777 mL
5 mM	0.2863 mL	1.4316 mL	2.8631 mL	14.3155 mL
10 mM	0.1432 mL	0.7158 mL	1.4316 mL	7.1578 mL
20 mM	0.0716 mL	0.3579 mL	0.7158 mL	3.5789 mL
50 mM	0.0286 mL	0.1432 mL	0.2863 mL	1.4316 mL
100 mM	0.0143 mL	0.0716 mL	0.1432 mL	0.7158 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

Keywords

transfectionnucleicLipidInhibitorinhibitDOTAP chlorideDOTAPCationic LipidCationicacid1,2-Dioleoyl-3-trimethylammonium-propane Chloride

Related Tags: DOTAP chloride chemical structure | DOTAP chloride in vitro | DOTAP chloride formula | DOTAP chloride molecular weight