DYKDDDDK tag Peptide [98849-88-8]

Référence A6002-25mg

Conditionnement : 25mg

Marque : APExBIO Technology

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DYKDDDDK tag Peptide

Catalog No.
A6002
FLAG peptide

Background

The FLAG tag Peptide (DYKDDDDK) is used as an epitope tag in recombinant protein expression to facilitate detection and purification.The FLAG tag Peptide is used to gently elute FLAG fusion proteins from anti-FLAG M1 and M2 affinity resins(K4628). The typical working concentration is 100 μg/ml. The FLAG tag Peptide will not elute 3X FLAG fusion proteins. For this application, please use the 3X FLAG Peptide(A6001).

References:
1. A. Einhauer, A. Jungbauer. The FLAG peptide, a versatile fusion tag for the purification of recombinant proteins. J. Biochem. Biophys. Methods 49 2001 455–465.
2. Hopp TP, Woods KR. Prediction of protein antigenic determinants from amino acid sequences. Proc Natl Acad Sci U S A 1981;78:3824–8.
3. Hopp TP. Protein surface analysis: methods for identifying antigenic determinants and other interaction sites. J Immunol Methods 1986;88:1–18.
4. Power BE, Ivancic N, Harley VR, Webster RG, Kortt AA, Irving RA, et al. High-level temperature-induced synthesis of an a

Product Citation

Chemical Properties

Physical AppearanceA solid
StorageDesiccate at -20°C
M.Wt1012.97
Cas No.98849-88-8
FormulaC41H60N10O20
SynonymsH-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-OH
Solubility≥50.65 mg/mL in DMSO; ≥210.6 mg/mL in H2O; ≥34.03 mg/mL in EtOH
Chemical NameDYKDDDDK Peptide
Canonical SMILESC1=CC(=CC=C1CC(C(=O)NC(CCCCN)C(=O)NC(CC(=O)O)C(=O)NC(CC(=O)O)C(=O)NC(CC(=O)O)C(=O)NC(CC(=O)O)C(=O)NC(CCCCN)C(=O)O)NC(=O)C(CC(=O)O)N)O
Shipping ConditionSmall Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips We do not recommend long-term storage for the solution, please use it up soon.

Biological Activity

Description DYKDDDDK tag Peptide is a 8 amino acid peptide with an enterokinase-cleavage site used for the purification of recombinant proteins.
Targets anti-DYKDDDDK M2 antibody          
IC50            

Quality Control

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