FAS siRNA (Rat)
Référence MBS828737-15nmol
Conditionnement : 15nmol
Marque : MyBiosource
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FAS siRNA – Tumor Necrosis Factor Receptor Superfamily Member 6 (Rat)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS828737MBS828737 is an siRNA targeting FAS. Also known as FAS siRNA (Rat). Reactive in Rat. Host: Synthetic. siRNA to inhibit FAS expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
FAS, siRNA
Full Product Name
FAS siRNA (Rat)
Product Gene Name
FAS sirna
Product Synonym Names
APT1; TNFRSF6; Tumor necrosis factor receptor superfamily member 6; Apo-1 antigen; Apoptosis-mediating surface antigen FAS; FASLG receptor; CD antigen CD95
Host
Synthetic
Species Reactivity
Rat
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
FAS siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
36,835 Da
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat FAS gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for FAS sirna
RNA Interference (RNAi)
Other Notes
Small volumes of FAS sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for FAS. It may not necessarily be applicable to this product.
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 6
NCBI Official Synonym Full Names
Fas cell surface death receptor
NCBI Official Symbol
Fas
NCBI Official Synonym Symbols
Tnfrsf6
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 6
UniProt Synonym Protein Names
Apo-1 antigen; Apoptosis-mediating surface antigen FAS; FASLG receptor; CD_antigen: CD95
Protein Family
Tumor necrosis factor receptor superfamily
UniProt Gene Name
Fas
UniProt Synonym Gene Names
Apt1; Tnfrsf6
UniProt Entry Name
TNR6_RAT
NCBI Protein Information
tumor necrosis factor receptor superfamily member 6
NCBI Summary for FAS
Tnfsf6/Fasl receptor [RGD, Feb 2006]
UniProt Comments for FAS
Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both ().
Show more Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with FAS sirna
| Reference Product | PubMed Publications |
|---|---|
| Faslg sirna | >3626 publications with FAS and Faslg |
| Casp8 sirna | >3027 publications with FAS and Casp8 |
| Fadd sirna | >1947 publications with FAS and Fadd |
| Bid sirna | >622 publications with FAS and Bid |
| Cflar sirna | >452 publications with FAS and Cflar |
| Daxx sirna | >101 publications with FAS and Daxx |
| Ptpn13 sirna | >78 publications with FAS and Ptpn13 |
| Tnfrsf1a sirna | >39 publications with FAS and Tnfrsf1a |
| Map3k5 sirna | >19 publications with FAS and Map3k5 |
Pathways associated with FAS sirna
| Products by Pathway | Pathway Diagram |
|---|---|
| Adipogenesis Pathway antibodies | Adipogenesis Pathway Diagram |
| African Trypanosomiasis Pathway antibodies | African Trypanosomiasis Pathway Diagram |
| African Trypanosomiasis Pathway antibodies | African Trypanosomiasis Pathway Diagram |
| Allograft Rejection Pathway antibodies | Allograft Rejection Pathway Diagram |
| Allograft Rejection Pathway antibodies | Allograft Rejection Pathway Diagram |
| Alzheimer's Disease Pathway antibodies | Alzheimer's Disease Pathway Diagram |
| Alzheimer's Disease Pathway antibodies | Alzheimer's Disease Pathway Diagram |
| Apoptosis Pathway antibodies | Apoptosis Pathway Diagram |
| Apoptosis Pathway antibodies | Apoptosis Pathway Diagram |
| Apoptosis Pathway antibodies | Apoptosis Pathway Diagram |
Diseases associated with FAS sirna
| Disease Name | Pubmed Publications |
|---|---|
| Necrosis Antibodies | >5080 publications with FAS and Necrosis |
| Autoimmune Diseases Antibodies | >2060 publications with FAS and Autoimmune Diseases |
| Inflammation Antibodies | >1845 publications with FAS and Inflammation |
| Nervous System Diseases Antibodies | >1589 publications with FAS and Nervous System Diseases |
| Disease Models, Animal Antibodies | >1527 publications with FAS and Disease Models, Animal |
| Liver Diseases Antibodies | >1495 publications with FAS and Liver Diseases |
| Fatty Liver Antibodies | >1059 publications with FAS and Fatty Liver |
| Hematologic Diseases Antibodies | >678 publications with FAS and Hematologic Diseases |
| Neoplasms, Experimental Antibodies | >652 publications with FAS and Neoplasms, Experimental |
| Heart Diseases Antibodies | >557 publications with FAS and Heart Diseases |
Organs/Tissues associated with FAS sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Lung Antibodies | >1249 publications with FAS and Lung |
| Thymus Antibodies | >425 publications with FAS and Thymus |
| Pancreas Antibodies | >378 publications with FAS and Pancreas |
| Embryonic Tissue Antibodies | >328 publications with FAS and Embryonic Tissue |
| Small Intestine Antibodies | >141 publications with FAS and Small Intestine |
| Uncharacterized Tissue Antibodies | >4 publications with FAS and Uncharacterized Tissue |