Principle of the Assay: This kit is a Enzyme-Linked Immunosorbent Assay (ELISA). Add samples to the pre-coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin-HRP is then added and then incubate. Unbound avidin-HRP is washed away during a washing step. TMB Substrate is then added and color develops. The reaction is stopped by addition of acidic stop solution and color changes into yellow that can be measured at 450 nm. The intensity of the color developed is inversely proportional to the concentration of COR in the sample. The concentration of COR in the sample is then determined by comparing the O.D. of the samples to the standard curve.

Intended Uses: This competitive ELISA kit is for the quantification of Cortisol(also known as COR) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.

Small volumes of COR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS1608240 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cortisol (COR) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing COR. The ELISA analytical biochemical technique of the MBS1608240 kit is based on COR antibody-COR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect COR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, COR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).