GFAP (Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein) (AP)
Référence MBS6124216-0,1mL
Conditionnement : 0.1mL
Marque : MyBiosource
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GFAP (Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein) (AP)
Formalin-paraffin human brain stained with GFAP Ab (SPM507). Note cytoplasmic staining.
Western blot analysis of GFAP in human brain lysate using GFAP Ab (SPM507).
GFAP Antibody – Mouse Monoclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6124216MBS6124216 is an antibody targeting GFAP. Also known as GFAP (Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein) (AP). Reactive in Bovine, Chicken, Human, Mouse, Porcine, Rabbit, Rat. Host: Mouse. More details
Product Overview
Online Datasheet in PDFProduct Name
GFAP, Monoclonal Antibody
Full Product Name
GFAP (Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein) (AP)
Product Gene Name
anti-GFAP antibody
Product Synonym Names
Astrocyte or Intermediate Filament Protein; Glial Fibrillary Acidic Protein
Clonality
Monoclonal
Isotype
IgG1
Clone Number
SPM507
Host
Mouse
Species Reactivity
Bovine, Chicken, Human, Mouse, Porcine, Rabbit, Rat
Form/Format
Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified by Protein A/G Affinity Chromatography.
Specificity
Recognizes GFAP. Species Crossreactivity: Human, Mouse, Rat, Bovine, Porcine, Rabbit, Chicken.
Preparation and Storage
May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of activity.
Molecular Weight
49,880 Da
Chromosome Location
Chromosome: 17; NC_000017.10 (42982994..42992920, complement). Location: 17q21
OMIM
137780
Immunogen
GFAP isolated from pig spinal cord. Cellular Localization: Cytoplasmic
Conjugate
AP
Positive Control
Brain or Astrocytoma
Product Categories/Family for anti-GFAP antibody
Antibodies; Growth Factors; Cytokines
Applications Tested/Suitable for anti-GFAP antibody
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-GFAP antibody
IP: 1-2ug/500ug protein lysate
IHC: 0.5-1ug/ml for 30 minutes at RT
IF: 1-2ug/ml
WB: 0.5-1ug/ml
Applications are based on unconjugated antibody.
IHC: 0.5-1ug/ml for 30 minutes at RT
IF: 1-2ug/ml
WB: 0.5-1ug/ml
Applications are based on unconjugated antibody.
Product References and Citations for anti-GFAP antibody
1. Herpers MJ et. Al. 1986, Acta Neuropathol, 70:333-339. 2. Van Muijen GN et. al. 1987, Lab Invest, 57:359-369. 3. Debus E, et. al. 1983, Differentiation, 25(2):193-203.
Other Notes
Small volumes of anti-GFAP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for GFAP. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
glial fibrillary acidic protein isoform 2
NCBI Official Synonym Full Names
glial fibrillary acidic protein
NCBI Official Symbol
GFAP
UniProt Protein Name
Glial fibrillary acidic protein
Protein Family
Glial fibrillary acidic protein
UniProt Gene Name
GFAP
UniProt Synonym Gene Names
GFAP
UniProt Entry Name
GFAP_HUMAN
NCBI Protein Information
glial fibrillary acidic protein
NCBI Summary for GFAP
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
Show more UniProt Comments for GFAP
GFAP: a class-III intermediate filament protein. A cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant isoform has been described, but its full length sequence has not been determined.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 17q21
Cellular Component: membrane; cytoplasm; intermediate filament; cytosol
Molecular Function: integrin binding; structural constituent of cytoskeleton; kinase binding
Biological Process: Bergmann glial cell differentiation; extracellular matrix organization and biogenesis; regulation of neurotransmitter uptake; response to wounding; neurite regeneration; intermediate filament organization; astrocyte development
Disease: Alexander Disease
Show more Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 17q21
Cellular Component: membrane; cytoplasm; intermediate filament; cytosol
Molecular Function: integrin binding; structural constituent of cytoskeleton; kinase binding
Biological Process: Bergmann glial cell differentiation; extracellular matrix organization and biogenesis; regulation of neurotransmitter uptake; response to wounding; neurite regeneration; intermediate filament organization; astrocyte development
Disease: Alexander Disease
Research Articles on GFAP
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with anti-GFAP antibody
| Reference Product | PubMed Publications |
|---|---|
| S100B antibody | >131 publications with GFAP and S100B |
| VIM antibody | >122 publications with GFAP and VIM |
| SRC antibody | >20 publications with GFAP and SRC |
| PRKCA antibody | >12 publications with GFAP and PRKCA |
| S100A1 antibody | >11 publications with GFAP and S100A1 |
| PSEN1 antibody | >8 publications with GFAP and PSEN1 |
| MEN1 antibody | >3 publications with GFAP and MEN1 |
Pathways associated with anti-GFAP antibody
| Products by Pathway | Pathway Diagram |
|---|---|
| Neural Crest Differentiation Pathway antibodies | Neural Crest Differentiation Pathway Diagram |
| Nuclear Signaling By ERBB4 Pathway antibodies | Nuclear Signaling By ERBB4 Pathway Diagram |
| Signal Transduction Pathway antibodies | Signal Transduction Pathway Diagram |
| Signaling By ERBB4 Pathway antibodies | Signaling By ERBB4 Pathway Diagram |
| Spinal Cord Injury Pathway antibodies | Spinal Cord Injury Pathway Diagram |
Diseases associated with anti-GFAP antibody
| Disease Name | Pubmed Publications |
|---|---|
| Nervous System Diseases Antibodies | >4342 publications with GFAP and Nervous System Diseases |
| Brain Diseases Antibodies | >3304 publications with GFAP and Brain Diseases |
| Gliosis Antibodies | >995 publications with GFAP and Gliosis |
| Cardiovascular Diseases Antibodies | >760 publications with GFAP and Cardiovascular Diseases |
| Brain Injuries Antibodies | >604 publications with GFAP and Brain Injuries |
| Drug Toxicity Antibodies | >533 publications with GFAP and Drug Toxicity |
| Nerve Degeneration Antibodies | >504 publications with GFAP and Nerve Degeneration |
| Necrosis Antibodies | >487 publications with GFAP and Necrosis |
| Inflammation Antibodies | >485 publications with GFAP and Inflammation |
| Epilepsy Antibodies | >323 publications with GFAP and Epilepsy |
Organs/Tissues associated with anti-GFAP antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Brain Antibodies | >7052 publications with GFAP and Brain |
| Nerve Antibodies | >3075 publications with GFAP and Nerve |
| Blood Antibodies | >1385 publications with GFAP and Blood |
| Spinal Cord Antibodies | >1196 publications with GFAP and Spinal Cord |
| Eye Antibodies | >899 publications with GFAP and Eye |
| Muscle Antibodies | >462 publications with GFAP and Muscle |
| Ganglia Antibodies | >253 publications with GFAP and Ganglia |
| Bone Marrow Antibodies | >222 publications with GFAP and Bone Marrow |
| Liver Antibodies | >182 publications with GFAP and Liver |
| Connective Tissue Antibodies | >161 publications with GFAP and Connective Tissue |