Glycogen Synthase Kinase 3, phosphorylated (Y279/Y216) (GSK3) (AP) (Drosophila)
Référence MBS6241537-0,1mL
Conditionnement : 0.1mL
Marque : MyBiosource
Glycogen Synthase Kinase 3, phosphorylated (Y279/Y216) (GSK3) (AP) (Drosophila)
Glycogen Synthase Kinase 3 (GSK3) Antibody – Mouse Monoclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6241537MBS6241537 is an antibody targeting GSK3. Also known as Glycogen Synthase Kinase 3, phosphorylated (Y279/Y216) (GSK3) (AP) (Drosophila). Reactive in Drosophila, Human, Mouse, Rat. Host: Mouse. More details
Product Overview
Online Datasheet in PDFProduct Name
Glycogen Synthase Kinase 3 (GSK3), Monoclonal Antibody
Full Product Name
Glycogen Synthase Kinase 3, phosphorylated (Y279/Y216) (GSK3) (AP) (Drosophila)
Product Gene Name
anti-GSK3 antibody
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
0.T.57
Host
Mouse
Species Reactivity
Drosophila, Human, Mouse, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Purity/Purification
Purified by Protein G Affinity Chromatography
Specificity
Recognizes Drosophila Glycogen Synthase Kinase 3, phosphorylated (Y279/Y216) (phosphoGSK-3) at 46kD (alpha) and 51kD (beta). Species Crossreactivity: mouse, rat, human, S. pombe and Dictyostelium. Expected to crossreact with nearly all species because of the extremely high conservation of this region among the known enzymes of many different species.
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
56,237 Da
Immunogen
Synthetic peptide (aa203-219, C-KQLLHGEPNVS[pY]ICSRY) corresponding to the catalytic domain (between subdomain VII & VIII) of Drosophila GSK-3/shaggy enzyme coupled to ovalbumin (Accession# NM_057366).
Conjugate
AP
Product Categories/Family for anti-GSK3 antibody
Antibodies; Protein Kinases
Applications Tested/Suitable for anti-GSK3 antibody
Western Blot (WB)
Application Notes for anti-GSK3 antibody
Not suitable for use in Immunoprecipitation.
Applications are based on unconjugated antibody.
WB: 0.5-2ug/ml detects phospho-GSK-3 in RIPA lysates from human Jurkat cells. Previous lots detected phospho-GSK-3 in mouse 3T3/A31, human A431 and rat L6 cell lysates. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with (HRP) goat anti-mouse and a chemiluminescence detection system
Applications are based on unconjugated antibody.
WB: 0.5-2ug/ml detects phospho-GSK-3 in RIPA lysates from human Jurkat cells. Previous lots detected phospho-GSK-3 in mouse 3T3/A31, human A431 and rat L6 cell lysates. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with (HRP) goat anti-mouse and a chemiluminescence detection system
Product References and Citations for anti-GSK3 antibody
1. Hughes K, et al. 1992. Eur. J. Biochem. 203:305-311. 2. Siddle K, et al. 1995. Biochem. J. 305:25-28. 3. Woodgett JR, et al. 1993. EMBO. 12:803-808. 4. Harwood AJ, et al. 1995. Cell. 80:139-148.
Other Notes
Small volumes of anti-GSK3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for GSK3. It may not necessarily be applicable to this product.
NCBI Official Full Name
gasket
NCBI Official Synonym Full Names
gasket
NCBI Official Symbol
gskt
NCBI Official Synonym Symbols
BcDNA:AT21229; BEST:GH16447; CG11338; CG31003; CT4237; Dm Gskt; DmelCG31003; Gsk3b; GSK3b; Gskt; mjl; NEST:bs22e06; NGSK
UniProt Protein Name
Putative glycogen synthase kinase-3 homolog
UniProt Synonym Protein Names
Protein gasket
Protein Family
Glycogen synthase kinase
UniProt Gene Name
gskt
UniProt Synonym Gene Names
GSK-3
NCBI Protein Information
CG31003 gene product from transcript CG31003-RA
Research Articles on GSK3
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Pathways associated with anti-GSK3 antibody
| Products by Pathway | Pathway Diagram |
|---|---|
| Circadian Rhythm - Fly Pathway antibodies | Circadian Rhythm - Fly Pathway Diagram |
| Hedgehog Signaling Pathway antibodies | Hedgehog Signaling Pathway Diagram |
| Hedgehog Signaling Pathway antibodies | Hedgehog Signaling Pathway Diagram |
| Wnt Signaling Pathway antibodies | Wnt Signaling Pathway Diagram |
| Wnt Signaling Pathway antibodies | Wnt Signaling Pathway Diagram |


