GreenSafe DNA Gel Stain

Référence E0207

Conditionnement : 1mL

Marque : Canvax Biotech

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Téléphone : +1 850 650 7790

GreenSafe DNA Gel Stain

Safe and sensitive alternative to Ethidium Bromide for DNA/RNA gel visualization in agarose and polyacrylamide gels.

The GreenSafe DNA Gel Stain is a safe and sensitive alternative to Ethidium Bromide (EtBr) for nucleic acid visualization. This DNA gel stain allows clear detection of double-stranded DNA, single-stranded DNA, and RNA in both agarose and polyacrylamide gels, providing superior sensitivity while eliminating toxic and mutagenic risks.

With a strong excitation peak at 490 nm and emission at 530 nm, the GreenSafe DNA Gel Stain delivers a superior signal-to-noise ratio and reduced background fluorescence. Compatible with common gel documentation systems, it enables seamless integration into existing workflows without the need for protocol changes.

Conveniently supplied as a 25,000× concentrated solution, it is suitable for both pre-staining and post-staining protocols, providing high sensitivity even for small DNA fragments.

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SKU: E0206 Categories: Loading Buffers & Staining, Electrophoresis

Detailed information:

Advantages & Features

  • Safe: non-toxic, non-mutagenic, non-carcinogenic.

  • Sensitive: detects small fragments with low background fluorescence.

  • Easy-to-use: replace Ethidium Bromide without changing imaging setup.

  • Versatile: compatible with agarose and polyacrylamide gels.

  • Reliable: lot-to-lot consistency ensured.

Specifications

  • Concentration:  25.000 X
  • Excitation spectra: 270-490 nm (Tree fluorescence excitation peaks: 270 nm,  295 nm and one strong excitation peak  490 nm
  • Excitation maximum, nm: ≈ 490 nm
  • Emission maximum, nm: ≈530 nm
  • Excitation light source:  UV

Includes

Includes for 1 mL:
– 1 mL GreenSafe DNA Gel Stain (sufficient up to 25 liters of agarose)

Download documentation

Applications

  • Safe alternative to Ethidium Bromide (EtBr).

  • Visualization of DNA and RNA in agarose and polyacrylamide gels.

  • Pre-staining or post-staining workflows.

  • Molecular cloning, genotyping, and sequencing workflows.

Tables & Figures

Quality Control

  • Validated for lot-to-lot reproducibility.

  • Tested for sensitivity and background reduction.

Advice

  • Protect from light to maintain stability.

  • Use gloves and standard lab PPE.

  • For optimal sensitivity, avoid overexposure during imaging.

Storage, Shipping & Guarantee

  • Shipped in: Ambient temperature.
  • Storage: between 2°C to 25°C protected from light. 4ºC recommended for long-term storage.

Citations

  • Mourabiti, F., Jouga, F., Mouzoun, Y., Arslan, S., Schena, R., Zouheir, Y., … & El Khalfi, B. (2025). Phenotypic and genotypic characterization of carbapenem encoding genes among carbapenem-resistant Gram-negative bacteria isolated from North Casablanca, MoroccoComparative Immunology, Microbiology and Infectious Diseases, 102399.
  • Dimov, D., Kostova, M., Vuchkov, A., Dimitrova, I., Kalaydzhiev, G., Staykova, G., … & Bozhilova, M. (2025). Genetic polymorphisms in Agouti signaling protein (ASIP) and melanocortin 1 receptor (MC1R) genes and their association with coat color in native Bulgarian sheep breedsSmall Ruminant Research, 107517.
  • Ghadamnan, Elmira. Characterization of Tick-Associated Microbiota in Spain Using a Nanopore-based Metabarcoding Approach: Insights into Potential Zoonotic Pathogens. 2023.
  • Díaz del Moral, S. (2023). Functions of the Wilms’ tumor suppressor gene (Wt1) in embryonic and adult myocardium.
  • PLANT, B. A. I. M. MALAYSIAN JOURNAL OF BIOCHEMISTRY & MOLECULAR BIOLOGY.
  • García-Sánchez, A. M., Miller, A. Z., Caldeira, A. T., & Cutillas, C. (2021). Bacterial communities from Trichuris spp. A contribution to deciphering the role of parasitic nematodes as vector of pathogens. Acta Tropica, 106277.
  • Fitriyah, F., Faramitha, Y., Sari, D. A., Kresnawaty, I., Panji, T., & Santoso, D. (2021, December). Improved direct lysis PCR amplification method of microalgal culture for sequencing and species identification. In IOP Conference Series: Earth and Environmental Science (Vol. 948, No. 1, p. 012013). IOP Publishing.
  • Pediconi, Natalia, et al. “Design and Synthesis of Piperazine-Based Compounds Conjugated to Humanized Ferritin as Delivery System of siRNA in Cancer Cells.” Bioconjugate Chemistry (2021).
  • Musso, N., Costantino, A., La Spina, S., Finocchiaro, A., Andronico, F., Stracquadanio, S., … & Emmanuele, G. (2020). New SARS-CoV-2 Infection Detected in an Italian Pet Cat by RT-qPCR from Deep Pharyngeal Swab. Pathogens9(9), 746.
  • Musso, N., Costantino, A., La Spina, S., Finocchiaro, A., Andronico, F., Stracquadanio, S., … & Emmanuele, G. (2020). New SARS-CoV-2 Infection in a Pet Cat with Severe Lung Disease in Italy.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Customers Review

Synonym(s)

DNA gel stain, Nucleic acid gel stain, DNA fluorescent stain, Agarose gel DNA stain, DNA visualization dye

Also known as:

  • Spanish: Tinte para gel de ADN, Colorante para ácidos nucleicos, Tinción fluorescente de ADN
  • French: Colorant pour gel d’ADN, Colorant pour acides nucléiques, Coloration fluorescente ADN
  • German: DNA-Gelfärbemittel, Nukleinsäure-Farbstoff, Fluoreszenzfarbstoff für DNA
  • Italian: Colorante per gel di DNA, Colorante per acidi nucleici, Colorazione fluorescente DNA

FAQs

Q: Is GreenSafe compatible with my existing gel documentation system?
A: Yes, it works with standard UV and blue-light imaging systems.

Q: Does it require protocol changes compared to Ethidium Bromide?
A: No, it can directly replace EtBr.

Q: Can it be used for both DNA and RNA?
A: Yes, it is validated for both dsDNA, ssDNA, and RNA.

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Référence
Description
Cond.
Prix HT
8378-SC
 100reactions