Guinea Pig Interferon Alpha (IFNa) ELISA Kit

Référence MBS2020416-24

Conditionnement : 24-Strip-Wells

Marque : MyBiosource

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Interferon Alpha (IFNa) ELISA Kit for Guinea Pig

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2020416
SPECIAL PROMOTION: Get FREE Starbucks gift card ( for 48-Wells-Kit or for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Alpha (IFNa). Standards or samples are then added to the appropriate microtiter plate… More details

Product Overview

Online Datasheet in PDF
Product Name
Interferon Alpha (IFNa), ELISA Kit
Full Product Name
Guinea Pig Interferon Alpha (IFNa) ELISA Kit
Product Gene Name
IFNa elisa kit
Product Synonym Names
IFNA1; IFL; LeIF D; IFN; IFN-Alpha; IFNA13; IFN-A; IFNAP22; IFN-Alpha 1b; Interferon Alpha 1b; Interferon, Leukocytic; IFN, Leukocyte; Interferon alpha-D
Species Reactivity
Guinea Pig
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Detection Range
15.6-1,000pg/mL
Sensitivity
5.8pg/mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Interferon Alpha (IFNa).
No significant cross-reactivity or interference between Interferon Alpha (IFNa) and analogues was observed.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Molecular Weight
22,319 Da
OMIM
147553
3D Structure
ModBase 3D Structure for P05000
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Interferon Alpha (IFNa) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Interferon Alpha (IFNa) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%

Product Categories/Family for IFNa elisa kit

Cytokine; Infection immunity
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of IFNa elisa kit

Guinea Pig IFNa ELISA Kit – Cat# MBS2020416

Other Notes

Small volumes of IFNa elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IFNa purchase

MBS2020416 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interferon Alpha (IFNa) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IFNa. The ELISA analytical biochemical technique of the MBS2020416 kit is based on IFNa antibody-IFNa antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IFNa antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IFNa. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for IFNa. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
interferon-alpha
NCBI Official Synonym Full Names
interferon, omega 1
NCBI Official Symbol
IFNW1
UniProt Protein Name
Interferon omega-1
UniProt Synonym Protein Names
Interferon alpha-II-1
UniProt Gene Name
IFNW1
UniProt Entry Name
IFNW1_HUMAN
NCBI Protein Information
interferon omega-1

UniProt Comments for IFNa

IFNW1: Belongs to the alpha/beta interferon family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 9p22

Cellular Component: extracellular space

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interferon-alpha/beta receptor binding; cytokine activity

Biological Process: B cell proliferation; adaptive immune response; response to exogenous dsRNA; cytokine and chemokine mediated signaling pathway; B cell differentiation; response to virus; regulation of MHC class I biosynthetic process; natural killer cell activation during immune response; innate immune response; cell cycle arrest; defense response to virus; humoral immune response; positive regulation of peptidyl-serine phosphorylation of STAT protein; T cell activation during immune response
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with IFNa elisa kit

 Reference Product  PubMed Publications
 IFNA2 elisa kit  >1 publications with IFNa and IFNA2
 MTAP elisa kit  >1 publications with IFNa and MTAP

Pathways associated with IFNa elisa kit

 Products by Pathway  Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 RIG-I-like Receptor Signaling Pathway antibodies  RIG-I-like Receptor Signaling Pathway Diagram
 RIG-I-like Receptor Signaling Pathway antibodies  RIG-I-like Receptor Signaling Pathway Diagram

Diseases associated with IFNa elisa kit

 Disease Name  Pubmed Publications
 Skin Neoplasms Antibodies  >1 publications with IFNa and Skin Neoplasms
 Carcinoma, Squamous Cell Antibodies  >1 publications with IFNa and Carcinoma, Squamous Cell
 Immune System Diseases Antibodies  >1 publications with IFNa and Immune System Diseases

Organs/Tissues associated with IFNa elisa kit

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >1 publications with IFNa and Lung