GW Blocking Peptide
Référence MBS8309588-5mg
Conditionnement : 5mg
Marque : MyBiosource
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Gw Blocking Peptide – >85% Purity
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8309588MBS8309588 is a blocking peptide targeting MASTL. Also known as GW Blocking Peptide. Reactive in Human. Host: Synthetic. The peptide is used to block Anti-GW Antibody reactivity. More details
Product Overview
Online Datasheet in PDFProduct Name
GW (MASTL), Blocking Peptide
Full Product Name
GW Blocking Peptide
Product Gene Name
MASTL blocking peptide
Product Synonym Names
GW; GWL; THC2; Serine/threonine-protein kinase greatwall; GW; GWL; hGWL; Microtubule-associated serine/threonine-protein kinase-like; MAST-L
Host
Synthetic
Species Reactivity
Human
Form/Format
Lyophilized Powder
Purity/Purification
>85%
Preparation and Storage
Store at -20 degree C for one year.
Molecular Weight
97,319 Da
Chromosome Location
Chromosome: 10; NC_000010.10 (27443753..27475848). Location: 10p12.1
OMIM
188000
Quality Control
The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
Directions for Use
Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4 degree C for overnight or at room temperature for 2 hours.
Applications Tested/Suitable for MASTL blocking peptide
Blocking (BL)
Other Notes
Small volumes of MASTL blocking peptide vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for MASTL. It may not necessarily be applicable to this product.
NCBI Official Full Name
serine/threonine-protein kinase greatwall isoform 1
NCBI Official Synonym Full Names
microtubule associated serine/threonine kinase-like
NCBI Official Symbol
MASTL
NCBI Official Synonym Symbols
GW; GWL; THC2; hGWL; MAST-L; GREATWALL; RP11-85G18.2
UniProt Protein Name
Serine/threonine-protein kinase greatwall
UniProt Synonym Protein Names
Microtubule-associated serine/threonine-protein kinase-like
Protein Family
Serine/threonine-protein kinase greatwall
UniProt Gene Name
MASTL
UniProt Synonym Gene Names
GW; GWL; THC2; GW; GWL; hGWL; MAST-L
UniProt Entry Name
GWL_HUMAN
NCBI Protein Information
serine/threonine-protein kinase greatwall; greatwall protein kinase; microtubule-associated serine/threonine-protein kinase-like
NCBI Summary for MASTL
This gene encodes a microtubule-associated serine/threonine kinase. Mutations at this locus have been associated with autosomal dominant thrombocytopenia, also known as thrombocytopenia-2. Alternatively spliced transcript variants have been described for this locus. [provided by RefSeq, Feb 2010]
UniProt Comments for MASTL
MASTL: Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation. Defects in MASTL are the cause of thrombocytopenia type 2 (THC2). Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Protein kinase, AGC; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; AGC group; MAST family
Chromosomal Location of Human Ortholog: 10p12.1
Cellular Component: nucleoplasm; centrosome; cytoplasm; nucleus; cleavage furrow
Molecular Function: protein serine/threonine kinase activity; protein phosphatase 2A binding; kinase activity; ATP binding
Biological Process: mitosis; regulation of cell cycle; cell division; mitotic cell cycle; G2/M transition of mitotic cell cycle; negative regulation of protein phosphatase type 2A activity; protein amino acid phosphorylation; response to DNA damage stimulus
Disease: Thrombocytopenia 2
Show more Protein type: Protein kinase, AGC; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; AGC group; MAST family
Chromosomal Location of Human Ortholog: 10p12.1
Cellular Component: nucleoplasm; centrosome; cytoplasm; nucleus; cleavage furrow
Molecular Function: protein serine/threonine kinase activity; protein phosphatase 2A binding; kinase activity; ATP binding
Biological Process: mitosis; regulation of cell cycle; cell division; mitotic cell cycle; G2/M transition of mitotic cell cycle; negative regulation of protein phosphatase type 2A activity; protein amino acid phosphorylation; response to DNA damage stimulus
Disease: Thrombocytopenia 2
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Pathways associated with MASTL blocking peptide
| Products by Pathway | Pathway Diagram |
|---|---|
| Cell Cycle Pathway antibodies | Cell Cycle Pathway Diagram |
| Cell Cycle, Mitotic Pathway antibodies | Cell Cycle, Mitotic Pathway Diagram |
| M Phase Pathway antibodies | M Phase Pathway Diagram |
| MASTL Facilitates Mitotic Progression Pathway antibodies | MASTL Facilitates Mitotic Progression Pathway Diagram |
| Mitotic Prophase Pathway antibodies | Mitotic Prophase Pathway Diagram |
Diseases associated with MASTL blocking peptide
| Disease Name | Pubmed Publications |
|---|---|
| Thrombocytopenia Antibodies | >3 publications with MASTL and Thrombocytopenia |
| Neoplasms Antibodies | >2 publications with MASTL and Neoplasms |
| Neoplasms, Experimental Antibodies | >1 publications with MASTL and Neoplasms, Experimental |
Organs/Tissues associated with MASTL blocking peptide
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Blood Antibodies | >2 publications with MASTL and Blood |
| Bone Antibodies | >1 publications with MASTL and Bone |
| Bone Marrow Antibodies | >1 publications with MASTL and Bone Marrow |
| Kidney Antibodies | >1 publications with MASTL and Kidney |
| Intestine Antibodies | >1 publications with MASTL and Intestine |
| Testis Antibodies | >1 publications with MASTL and Testis |