Conditionnement : 1mg
Téléphone : +1 850 650 7790
Synonyms:
| Description | Harringtonine is a naturally occurring alkaloid that inhibits DNA synthesis and protein synthesis and strongly kills S-phase cells. Harringtonine has antitumor and antiviral activities. |
| Targets & IC50 | HeLa cells:4 μM, HSV1:0.1081-0.1584 μM, CHIKV:0.24 μM (EC50), HL-60 cells:0.047 μM, BHK-21 cells:0.24 μM (EC50) |
| In vitro | Harringtonine inhibits the elongation phase of translation by preventing substrate binding to the acceptor site on the 60-S ribosome subunit, blocking aminoacyl-tRNA binding and peptide bond formation [1]. It displays potent inhibition of Chikungunya virus infection (EC50: 0.24 μM) and could inhibit other alphaviruses [2]. Additionally, Harringtonine inhibits the growth of human myeloid leukemia cells in vitro at low concentrations [3]. |
| In vivo | METHODS: To study in vivo activity, Harringtonine (0.5-4 mg/mouse) was injected retroorbital into C57BL/6 mice. RESULTS: Within 5 min, Harringtonine completely depleted multimers in all organs except testis and brain, and it took up to 30 min to significantly reduce the number of multimers due to the blood-brain and blood-testis barriers. [3] |
| Molecular Weight | 531.59 |
| Formula | C28H37NO9 |
| Cas No. | 26833-85-2 |
| Smiles | COC(=O)C[C@](O)(CCC(C)(C)O)C(=O)O[C@H]1[C@H]2c3cc4OCOc4cc3CCN3CCC[C@]23C=C1OC |
| Relative Density. | 1.35 g/cm3 |
| Storage | Keep away from direct sunlight,Store under nitrogen,Keep away from moisture Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. | |||||||||||||||||||||||||||||||||||
| Solubility Information | DMSO: 55 mg/mL (103.46 mM), Sonication is recommended.  | |||||||||||||||||||||||||||||||||||
| In Vivo Formulation | 10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (3.76 mM), Sonication is recommended. Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions. | |||||||||||||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||||||||||||
DMSO
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | ||||||||||||||||||||||||||||||||||||
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
