Hematoxylin, PROBE
Référence MBS258481-5x250mL
Conditionnement : 5x250mL
Marque : MyBiosource
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Hematoxylin, PROBE Reagent
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS258481This uniquely formulated Probe hematoxylin is an excellent counterstain for in situ DNA hybridization procedure. Probe hematoxylin produces clean blue color of nuclei on formalin-fixed paraffin-embedded (FFPE) tissue sections and frozen sections and is stable… More details
Product Overview
Online Datasheet in PDFProduct Name
Hematoxylin, PROBE, Reagent
Full Product Name
Hematoxylin, PROBE
Notes
Ready to use counterstain for in situ hybridization
Product Categories/Family for Hematoxylin, PROBE reagent
IHC Reagents
Applications Tested/Suitable for Hematoxylin, PROBE reagent
Immunohistochemistry (IHC)
Application Notes for Hematoxylin, PROBE reagent
1. After chromogen staining, wash slide 2-3X with de/di water, 2X with
2. Apply Probe hematoxylin for 2-3 minutes; wash with tap water 5-7X.
3. Soak the slides in Immuno Automation buffer (AR-6561), PBS or any buffer with pH 7.4-8 for 2-3 minutes. Hematoxylin changes color from purple red to blue.
4. Wash slide 2-3X with de/di water.
5. The slides can be mounted with aqueous mounting medium (ImmunoHistoMount (AR-6503) or equivalent) for chromogens like AEC, DAB, Fast red, BCIP/INT, or Super Fast red. DAB and Super Fast can be mounted with Organic mounting mediums like Organo mount/limonene (AR-6504) or equivalent after dehydrating the tissue with alcohols and organic solvents.
2. Apply Probe hematoxylin for 2-3 minutes; wash with tap water 5-7X.
3. Soak the slides in Immuno Automation buffer (AR-6561), PBS or any buffer with pH 7.4-8 for 2-3 minutes. Hematoxylin changes color from purple red to blue.
4. Wash slide 2-3X with de/di water.
5. The slides can be mounted with aqueous mounting medium (ImmunoHistoMount (AR-6503) or equivalent) for chromogens like AEC, DAB, Fast red, BCIP/INT, or Super Fast red. DAB and Super Fast can be mounted with Organic mounting mediums like Organo mount/limonene (AR-6504) or equivalent after dehydrating the tissue with alcohols and organic solvents.
Other Notes
Small volumes of Hematoxylin, PROBE reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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MBS258481 is a reagent targeting Hematoxylin, PROBE.