Dot blot analysis of 0.2 - 100 pmol of the peptides containing other modifications of histone H3 and the unmodified H3K9 sequence using GTX60357 Histone H3K9me2 (Di-methyl Lys9) antibody - ChIP grade.
Dilution : 1:20,000

ICC/IF analysis of 4% paraformaldehyde fixed NIH3T3 cells using GTX60357 Histone H3K9me2 (Di-methyl Lys9) antibody - ChIP grade.
Green : Primary antibody
Blue : DAPI
Dilution : 1:500

ELISA analysis of peptide containing the histone modification of interest using GTX60357 Histone H3K9me2 (Di-methyl Lys9) antibody - ChIP grade.

ChIP analysis of sheared chromatin from 10⁶ HeLa cells using GTX60357 Histone H3K9me2 (Di-methyl Lys9) antibody - ChIP grade. A titration of the antibody consisting of 1, 2, 5, and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers specific for promoter of the inactive HBB gene and the coding region of the inactive MYOD gene, used as positive controls, and for the promoters of the active genes c-fos and GAPDH, used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

WB analysis of histone extracts (15 μg) from HeLa cells using GTX60357 Histone H3K9me2 (Di-methyl Lys9) antibody - ChIP grade.
Dilution : 1:1,000