HP siRNA (Mouse)
Référence MBS828993-15nmol
Conditionnement : 15nmol
Marque : MyBiosource
Contactez votre distributeur local :
Téléphone : +1 850 650 7790
HP siRNA – Haptoglobin (Mouse)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS828993MBS828993 is an siRNA targeting HP. Also known as HP siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit HP expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
HP, siRNA
Full Product Name
HP siRNA (Mouse)
Product Gene Name
HP sirna
Product Synonym Names
Haptoglobin; Zonulin
Host
Synthetic
Species Reactivity
Mouse
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
HP siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
38,752 Da
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse HP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for HP sirna
RNA Interference (RNAi)
Other Notes
Small volumes of HP sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for HP. It may not necessarily be applicable to this product.
NCBI Official Full Name
haptoglobin
NCBI Official Synonym Full Names
haptoglobin
NCBI Official Symbol
Hp
NCBI Official Synonym Symbols
HP-1; preHP2
UniProt Protein Name
Haptoglobin
UniProt Synonym Protein Names
Zonulin
Protein Family
HpaII very short patch repair endonuclease
UniProt Gene Name
Hp
UniProt Entry Name
HPT_MOUSE
NCBI Protein Information
haptoglobin
UniProt Comments for HP
HPR: a haptoglobin-related protein that binds hemoglobin as efficiently as haptoglobin. Unlike haptoglobin, plasma concentration of this protein is unaffected in patients with sickle cell anemia and extensive intravascular hemolysis, suggesting a difference in binding between haptoglobin-hemoglobin and haptoglobin-related protein-hemoglobin complexes to CD163, the hemoglobin scavenger receptor. This protein may also be a clinically important predictor of recurrence of breast cancer. [provided by RefSeq, Oct 2011]
Protein type: Secreted, signal peptide; Secreted
Cellular Component: Golgi apparatus; extracellular space; endoplasmic reticulum; extracellular region
Molecular Function: antioxidant activity; protein homodimerization activity; hemoglobin binding; serine-type endopeptidase activity; catalytic activity
Biological Process: response to hydrogen peroxide; immune system process; defense response to bacterium; negative regulation of oxidoreductase activity; acute-phase response; proteolysis; liver development
Show more Protein type: Secreted, signal peptide; Secreted
Cellular Component: Golgi apparatus; extracellular space; endoplasmic reticulum; extracellular region
Molecular Function: antioxidant activity; protein homodimerization activity; hemoglobin binding; serine-type endopeptidase activity; catalytic activity
Biological Process: response to hydrogen peroxide; immune system process; defense response to bacterium; negative regulation of oxidoreductase activity; acute-phase response; proteolysis; liver development
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with HP sirna
| Reference Product | PubMed Publications |
|---|---|
| Cd163 sirna | >62 publications with HP and Cd163 |
| Apoa1 sirna | >13 publications with HP and Apoa1 |
Pathways associated with HP sirna
| Products by Pathway | Pathway Diagram |
|---|---|
| Binding And Uptake Of Ligands By Scavenger Receptors Pathway antibodies | Binding And Uptake Of Ligands By Scavenger Receptors Pathway Diagram |
| Scavenging Of Heme From Plasma Pathway antibodies | Scavenging Of Heme From Plasma Pathway Diagram |
| Vesicle-mediated Transport Pathway antibodies | Vesicle-mediated Transport Pathway Diagram |
Diseases associated with HP sirna
| Disease Name | Pubmed Publications |
|---|---|
| Cardiovascular Diseases Antibodies | >1727 publications with HP and Cardiovascular Diseases |
| Inflammation Antibodies | >1201 publications with HP and Inflammation |
| Hypersensitivity Antibodies | >672 publications with HP and Hypersensitivity |
| Hypertension Antibodies | >528 publications with HP and Hypertension |
| Autoimmune Diseases Antibodies | >507 publications with HP and Autoimmune Diseases |
| Kidney Diseases Antibodies | >494 publications with HP and Kidney Diseases |
| Necrosis Antibodies | >492 publications with HP and Necrosis |
| Fibrosis Antibodies | >469 publications with HP and Fibrosis |
| Arthritis Antibodies | >327 publications with HP and Arthritis |
| Leukemia Antibodies | >286 publications with HP and Leukemia |
Organs/Tissues associated with HP sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Blood Antibodies | >5771 publications with HP and Blood |
| Liver Antibodies | >1298 publications with HP and Liver |
| Lung Antibodies | >1207 publications with HP and Lung |
| Bone Antibodies | >892 publications with HP and Bone |
| Muscle Antibodies | >769 publications with HP and Muscle |
| Kidney Antibodies | >728 publications with HP and Kidney |
| Skin Antibodies | >590 publications with HP and Skin |
| Intestine Antibodies | >505 publications with HP and Intestine |
| Joint Antibodies | >341 publications with HP and Joint |
| Connective Tissue Antibodies | >339 publications with HP and Connective Tissue |