RayBio® C-Series Human Cytokine Antibody Array 2000 Kit. A combination of Human Cytokine Antibody Array C6, C7, & C8. Detects 174 Human Cytokines. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 174 |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| Adiponectin (ACRP30)
| Activin A
| AgRP
| ALCAM (CD166)
| Amphiregulin
|
| Angiogenin
| Angiopoietin-2
| Axl
| CD80 (B7-1)
| BDNF
|
| bFGF
| BLC (CXCL13)
| BMP-4
| BMP-5
| BMP-6
|
| BMP-7
| beta-NGF
| Betacellulin (BTC)
| Cardiotrophin-1 (CT-1)
| CCL28 (MEC)
|
| CD14
| Ck beta 8-1 (CCL23)
| CNTF
| CTACK (CCL27)
| CXCL16
|
| DR6 (TNFRSF21)
| Dtk
| EGF
| EGFR
| ENA-78 (CXCL5)
|
| Endoglin (CD105)
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
| Eotaxin-3 (CCL26)
| ErbB3
|
| E-Selectin
| Fas (TNFRSF6/Apo-1)
| Fas Ligand (TNFSF6)
| FGF-4
| FGF-6
|
| FGF-7 (KGF)
| FGF-9
| Flt-3 Ligand
| Fractalkine (CX3CL1)
| GCP-2 (CXCL6)
|
| GCSF
| GDNF
| GITR (TNFRSF18)
| GITR Ligand (TNFSF18)
| GM-CSF
|
| GRO alpha/beta/gamma
| GRO alpha (CXCL1)
| HCC-4 (CCL16)
| HGF
| I-309 (TCA-3/CCL1)
|
| ICAM-1 (CD54)
| ICAM-2 (CD102)
| ICAM-3 (CD50)
| IFN-gamma
| IGFBP-1
|
| IGFBP-2
| IGFBP-3
| IGFBP-4
| IGFBP-6
| IGF-1
|
| IGF-1 R
| IGF-2
| IL-1 R2
| IL-1 R4 (ST2)
| IL-1 R1
|
| IL-10
| IL-10 R beta
| IL-11
| IL-12 p40
| IL-12 p70
|
| IL-13
| IL-13 R alpha 2
| IL-15
| IL-16
| IL-17A
|
| IL-18 BP alpha
| IL-18 R beta (AcPL)
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-1 ra (IL-1 F3)
|
| IL-2
| IL-2 R beta (CD122)
| IL-2 R gamma (Common gamma Chain)
| IL-2 R alpha
| IL-21 R
|
| IL-3
| IL-4
| IL-5
| IL-5 R alpha
| IL-6
|
| IL-6 R
| IL-7
| IL-8 (CXCL8)
| IL-9
| IP-10 (CXCL10)
|
| I-TAC (CXCL11)
| LAP/TGF beta 1
| Leptin
| Leptin R
| LIF
|
| Light (TNFSF14)
| L-Selectin (CD62L)
| Lymphotactin (XCL1)
| MCP-1 (CCL2)
| MCP-2 (CCL8)
|
| MCP-3 (MARC/CCL7)
| MCP-4 (CCL13)
| M-CSF
| M-CSF R
| MDC (CCL22)
|
| MIF
| MIG (CXCL9)
| MIP-1 alpha (CCL3)
| MIP-1 beta (CCL4)
| MIP-1 delta (CCL15)
|
| MIP-3 alpha (CCL20)
| MIP-3 beta (CCL19)
| MMP-1
| MMP-13
| MMP-3
|
| MMP-9
| MPIF-1 (CCL23)
| MSP alpha/beta
| NAP-2 (PPBP/CXCL7)
| NGFR (TNFRSF16)
|
| NT-3
| NT-4
| Oncostatin M
| Osteoprotegerin (TNFRSF11B)
| PARC (CCL18)
|
| PDGF-AA
| PDGF R alpha
| PDGF R beta
| PDGF-AB
| PDGF-BB
|
| PECAM-1 (CD31)
| PLGF
| Prolactin
| RANTES (CCL5)
| SCF
|
| SCF R (CD117/c-kit)
| SDF-1 alpha (CXCL12 alpha)
| SDF-1 beta (CXCL12 beta)
| gp130
| Siglec-5 (CD170)
|
| TNF RII (TNFRSF1B)
| TNF RI (TNFRSF1A)
| TARC (CCL17)
| TECK (CCL25)
| TGF beta 2
|
| TGF alpha
| TGF beta 3
| TGF beta 1
| Thrombopoietin (TPO)
| Tie-1
|
| Tie-2
| TIMP-1
| TIMP-2
| TIMP-4
| TNF alpha
|
| TNF beta (TNFSF1B)
| TRAIL R3 (TNFRSF10C)
| TRAIL R4 (TNFRSF10D)
| uPAR
| VE-Cadherin (CDH5)
|
| VEGF-A
| VEGFR2
| VEGFR3
| VEGF-D
| |
Species Detected
Human
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Cytokine Antibody Array C2000 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
Excel (AAH-CYT-6_Analysis_Tool.xls)
Excel (AAH-CYT-7_Analysis_Tool.xls)
Excel (AAH-CYT-8_Analysis_Tool.xls)
citations
It is not waste if it is therapy: cellular, secretory and functional properties of reamer-irrigator-aspirator (RIA)-derived autologous bone grafts
- Species:
- Human
- Sample Type:
- Other / Unspecified (liquid RIA samples)
- Year:
- 2025
- Journal Name:
- Journal of orthopaedics and traumatology : official journal of the Italian Society of Orthopaedics and Traumatology
- PubMed ID:
- 40140186
Discovery of a transdermally deliverable pentapeptide for activating AdipoR1 to promote hair growth
- Species:
- Human
- Sample Type:
- Serum (concentrated serum-free medium from human DP cells)
- Year:
- 2021
- Journal Name:
- EMBO Molecular Medicine
- PubMed ID:
- 34486824
Tumor specific cytokine pattern explored in renal cell carcinoma
- Species:
- Human
- Sample Type:
- Tissue Lysate / Homogenate (tissue homogenates normal, para-cancerous and tumor RCC tissue)
- Year:
- 2026
- Journal Name:
- PLoS ONE
- PubMed ID:
- 42809553
Notch3 promotes prostate cancer-induced bone lesion development via MMP-3
- Species:
- Human
- Sample Type:
- Tissue Lysate / Homogenate (lysates of subcutaneous, tibial tumors)
- Year:
- 2020
- Journal Name:
- Oncogene
- PubMed ID:
- 31467432
Xenografting of human umbilical mesenchymal stem cells from Wharton's jelly ameliorates mouse spinocerebellar ataxia type 1
- Species:
- Human
- Sample Type:
- Conditioned Media
- Year:
- 2019
- Journal Name:
- Translational neurodegeneration
- PubMed ID:
- 31508229
Reversal of bleomycin-induced rat pulmonary fibrosis by a xenograft of human umbilical mesenchymal stem cells from Wharton's jelly
- Species:
- Human
- Sample Type:
- Tissue Lysate (mesenchymal tissue from human umbilical cords)
- Year:
- 2019
- Journal Name:
- Theranostics
Production enhancement of human adipose-derived mesenchymal stem cells by low-intensity ultrasound stimulation
- Species:
- Human
- Sample Type:
- human adipose-derived mesenchymal stem cells (hAdMSCs)
- Year:
- 2022
- Journal Name:
- Scientific reports
TRPC3 shapes the ER-mitochondria Ca2+ transfer characterizing tumour-promoting senescence
- Species:
- Human
- Year:
- 2022
Profiling the 3D interaction between germ cell tumors and microenvironmental cells at the transcriptome and secretome level
- Species:
- Human
- Sample Type:
- TCam-2 cell, 2102EP cell, JAR cell, 1411H cell, THP-1 cell, JURKAT cell, HUVEC, MPAF cell
- Year:
- 2022
- Journal Name:
- Molecular Oncology
Extracellular glucose triggers metabolic reprogramming of cultured human bronchial epithelial cells and indirect fibroblast activation
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (conditioned media from HBECs)
- Year:
- 2024
- Journal Name:
- FEBS Open Bio
- PubMed ID:
- 38952051



