AAH-PER-1-4 | Human EGFR Phosphorylation Array C1 Biovalley

RayBiotech's EGFR Phosphorylation Antibody Array revealed key EGFR phosphorylation sites in carcinoma arising from inverted papilloma. Figure adapted from Hirakawa et al., Journal of Personalized Medicine, 2023.

Human EGFR Phosphorylation Array C1

C-Series Human EGFR Phosphorylation Antibody Array 1 Kit. Detects 16 site specific and pan Human EGFRs. Suitable for all liquid sample types but intended for use with cell and tissue lysates.

Product Description

Specifications

Size	2 Sample Kit, 4 Sample Kit, 8 Sample Kit
Species	Human
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-Quantitative	Semi-Quantitative
Number of Targets Detected	16
Recommended Applications	Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Solid Support	Membrane
Design Principle	Sandwich-based
Method Of Detection	Chemiluminescence
Research Area	Cancer, Post-Translational Modifications, Phosphorylation, HER/ErbB Signaling
Shipping Type	Blue ice
Storage	-20°C

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
EGFR (P-Tyr845) Gene Symbol: EGFR Gene ID: 1956 Accession #: P00533	EGFR (P-Tyr992) Gene Symbol: EGFR Gene ID: 1956 Accession #: P00533 ELISA Kit: RayBio Human Phospho-EGFR (Y992) ELISA	EGFR (P-Tyr1045) Gene Symbol: EGFR Gene ID: 1956 Accession #: P00533 ELISA Kit: RayBio Human Phospho-EGFR (Y1045) ELISA	EGFR (P-Tyr1068) Gene Symbol: EGFR Gene ID: 1956 Accession #: P00533 ELISA Kit: RayBio Human Phospho-EGFR (Y1068) ELISA	EGFR (P-Tyr1086) Gene Symbol: EGFR Gene ID: 1956 Accession #: P00533 ELISA Kit: RayBio Human Phospho-EGFR (Y1086) ELISA
EGFR (P-Tyr1148) Gene Symbol: EGFR Gene ID: 1956 Accession #: P00533	EGFR (P-Tyr1173) Gene Symbol: EGFR Gene ID: 1956 Accession #: P00533	EGFR (P-Ser1046/1047) Gene Symbol: EGFR Gene ID: 1956 Accession #: P00533	ErbB2 (P-Tyr877) Gene Symbol: ErbB2 Gene ID: 2064 Accession #: P04626	ErbB2 (P-Tyr1112) Gene Symbol: ErbB2 Gene ID: 2064 Accession #: P04626
ErbB2 (P-Tyr 1221/1222) Gene Symbol: ErbB2 Gene ID: 2064 Accession #: P04626	ErbB2 (P-Tyr1248) Gene Symbol: ErbB2 Gene ID: 2064 Accession #: P04626	ErbB2 (P-Thr686) Gene Symbol: ErbB2 Gene ID: 2064 Accession #: P04626	ErbB2 (P-Ser1113) Gene Symbol: ErbB2 Gene ID: 2064 Accession #: P04626	ErbB3 (P-Tyr1289) Gene Symbol: ErbB3 Gene ID: 2065 Accession #: P21860
ErbB4 (P-Tyr1284) Gene Symbol: ErbB4 Gene ID: 2066 Accession #: Q15303

Typical Data

RayBiotech's EGFR Phosphorylation Antibody Array revealed key EGFR phosphorylation sites in carcinoma arising from inverted papilloma. Figure adapted from Hirakawa et al., Journal of Personalized Medicine, 2023.

RayBiotech's EGFR Phosphorylation Antibody Array revealed EGFR and ErbB2 activation in B-cell lymphoma cells stimulated with HIV-1 p17 variants. Figure adapted from Giagulli et al., Cancer Gene Therapy, 2021.

RayBiotech's EGFR Phosphorylation Antibody Array revealed reduced EGFR-Y845 phosphorylation in pancreatic cancer cells and tumors treated with metformin. Figure adapted from Kato et al., Oncology Reports, 2016.

RayBiotech's EGFR Phosphorylation Antibody Array revealed SRC-mediated phosphorylation of EGFR-Y845 in ovarian cancer cells stimulated with lysophosphatidic acid. Figure adapted from Panupinthu et al., Oncogene, 2013.

Kit Components

Human EGFR Phosphorylation Antibody Array C1 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
Protease Inhibitor Cocktail
Phosphatase Inhibitor Cocktail Set I
Phosphatase Inhibitor Cocktail II
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Additional Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Assay Procedure Summary

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

documents

Datasheet (AAH-PER-1.pdf)

SDS (AAH-PER-1.pdf)

Excel (AAH-PER-1_Analysis_Tool.xls)

citations

Human Papillomavirus Infection and EGFR Exon 20 Insertions in Sinonasal Inverted Papilloma and Squamous Cell Carcinoma
Species:
Human
Sample Type:
inverted papilloma, squamous cell carcinoma
Year:
2023
Journal Name:
Journal of Personalized Medicine
HER2 amplification in tumors activates PI3K/Akt signaling independent of HER3
Species:
Human
Sample Type:
Cell Lysate (Breast cancer model of Her2 signaling importance)
Year:
2018
Journal Name:
Cancer Research
Identification and therapeutic intervention of coactivated anaplastic lymphoma kinase, fibroblast growth factor receptor 2, and ephrin type-A receptor 5 kinases in hepatocellular carcinoma
Species:
Human
Sample Type:
Cell Lysate (Heptocellular carnoma cell lines SMMC-7721 and others)
Year:
2018
Journal Name:
Hepatology
PubMed ID:
29356025
B-cell clonogenic activity of HIV-1 p17 variants is driven by PAR1-mediated EGF transactivation
Species:
Human
Sample Type:
Cell Lysate
Year:
2020
Journal Name:
Cancer Gene Therapy
Afatinib Overcomes Pemetrexed-Acquired Resistance in Non-Small Cell Lung Cancer Cells Harboring an EML4-ALK Rearrangement
Year:
2019
Journal Name:
Cells
PubMed ID:
31795298
Trastuzumab, but Not Pertuzumab, Dysregulates HER2 Signaling to Mediate Inhibition of Autophagy and Increase in Reactive Oxygen Species Production in Human Cardiomyocytes
Species:
Human
Sample Type:
Cell Lysate (Human Primary Cardiomyocytes)
Year:
2016
Journal Name:
Molecular Cancer Therapeutics
PubMed ID:
27197303
Self-reinforcing loop of amphiregulin and Y-box binding protein-1 contributes to poor outcomes in ovarian cancer
Species:
Human
Sample Type:
Cell Lysate (CAOV3 Cells)
Year:
2014
Journal Name:
Oncogene
Peiminine serves as an adriamycin chemosensitizer in gastric cancer by modulating the EGFR/FAK pathway
Species:
Human
Sample Type:
Cell Lysate (Gastric Cancer cell lines treated with adriamycin)
Year:
2018
Journal Name:
Oncology Reports
PubMed ID:
29328433
A Monoclonal Antibody to ADAM17 Inhibits Tumor Growth by Inhibiting EGFR and Non-EGFR-Mediated Pathways
Species:
Human
Sample Type:
Tissue Lysate (Human tumor cells xenograft models)
Year:
2015
Journal Name:
Molecular Cancer Therapeutics
PubMed ID:
25948294
DARPP-32 Increases Interactions Between Epidermal Growth Factor Receptor and ERBB3 to Promote Tumor Resistance to Gefitinib
Species:
Human
Sample Type:
Cell Lysate (MKN-28 gastric cancer cell lines)
Year:
2011
Journal Name:
Gastroenterology

Human EGFR Phosphorylation Array C1

Référence AAH-PER-1-4

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Human EGFR Phosphorylation Array C1