RayBio® Human GDNF ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Human GDNF ELISA Kit
Human GDNF ELISA Kit
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 4 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 4 pg/ml - 2000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P39905 |
| Gene Id | 2668 |
| Gene Symbols | GDNF |
| Protein Name / Synonyms | Glial cell line-derived neurotrophic factor (hGDNF) (Astrocyte-derived trophic factor) (ATF) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Human GDNF qIPCR ELISA Kit
As low as $639.00
RayPlex® Human GDNF Bead Assay
As low as $240.00
Target Background
Glial cell line-derived neurotrophic factor, a member of the transforming growth factor-beta superfamily is a survival factor for midbrain dopaminergic neurons, sympathetic and spinal motor neurons. It is also required for the development of the enteric nervous system, ureters and kidneys. Members of the GDNF family include neurturin, artemin and persephin, all of which have their selective receptors that bind to a common transmembrane receptor tyrosine kinase (RET) to induce signaling. Parkinson's disease is a candidate for GDNF-based treatment; however, it is also been tested for stroke and motor neuron injury.
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human GDNF ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified GDNF, including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Dental Pulp-Derived Stem Cells Preserve Astrocyte Health During Induced Gliosis by Modulating Mitochondrial Activity and Functions
- Species:
- Human
- Sample Type:
- dental pulp-derived stem cells (DPSCs)
- Year:
- 2022
- Journal Name:
- Cellular and Molecular Neurobiology
Glial cell line-derived neurotrophic factor (GDNF) in blood serum and lacrimal fluid of patients with a current depressive episode
- Species:
- Human
- Sample Type:
- lacrimal fluid, serum
- Year:
- 2022
- Journal Name:
- Journal of Affective Disorders
Therapeutic Effect of Human Mesenchymal Stem Cell-Conditioned Medium on Erectile Dysfunction
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (CM mesenchymal stem cell-conditioned medium)
- Year:
- 2022
- Journal Name:
- The world journal of men's health
- PubMed ID:
- 35021313
Lineage-Specific Commitment of Stem Cells with Organic and Graphene Oxide-Functionalized Nanofibers
- Species:
- Human
- Sample Type:
- Conditioned Media (Dental Pulp stem cells)
- Year:
- 2018
- Journal Name:
- Advances in simulation (London, England)
Expansion of Human Limbal Epithelial Stem/Progenitor Cells Using Different Human Sera: A Multivariate Statistical Analysis
- Species:
- Human
- Sample Type:
- Serum (blood-derived preparations human sera)
- Year:
- 2020
- Journal Name:
- International Journal of Molecular Sciences
- PubMed ID:
- 32854428
Human Muscle Progenitor Cells Overexpressing Neurotrophic Factors Improve Neuronal Regeneration in a Sciatic Nerve Injury Mouse Model
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (cell supernatant of transduced hMPC)
- Year:
- 2019
- Journal Name:
- Frontiers in Neuroscience
- PubMed ID:
- 30872995
Transport of Glial Cell Line-Derived Neurotrophic Factor into Liposomes across the Blood-Brain Barrier: In Vitro and in Vivo Studies
- Species:
- Human
- Sample Type:
- Serum (serum, detecting GDNF liposomes); Tissue Lysate
- Year:
- 2014
- Journal Name:
- International Journal of Molecular Sciences
- PubMed ID:
- 24583850
Analgesia Targeting IB4-Positive Neurons in Cancer-Induced Mechanical Hypersensitivity .The Journal of Pain
- Species:
- Human
- Sample Type:
- Conditioned Media (HSC-3 Cells and Normal Oral Keratinocytes cell supernate)
- Year:
- 2012
- Journal Name:
- The Journal of Pain
Human bone marrow mesenchymal stem cells protect catecholaminergic and serotonergic neuronal perikarya and transporter function from oxidative stress by the secretion of glial-derived neurotrophic factor
- Species:
- Human
- Sample Type:
- Conditioned Media (Conditioned Media); Conditioned Media (MSC stem cells from various pasages)
- Year:
- 2012
- Journal Name:
- Brain Research
- PubMed ID:
- 22143094
Neurotrophic activity of human adipose stem cells isolated from deep and superficial layers of abdominal fat
- Species:
- Human
- Sample Type:
- Conditioned Media (Adipose-derived Stem Cell supernate)
- Year:
- 2011
