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> Human IgG2 (F(ab)2 specific) Mouse Monoclonal Antibody [Clone ID: NI 6014 (HP 6014)]

Human IgG2 (F(ab)2 specific) Mouse Monoclonal Antibody [Clone ID: NI 6014 (HP 6014)]

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Référence AM20253TC-N

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Human IgG2 (F(ab)2 specific) Mouse Monoclonal Antibody [Clone ID: NI 6014 (HP 6014)]

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SKU
AM20253TC-N
Human IgG2 (F(ab)2 specific) mouse monoclonal antibody, clone NI 6014 (HP 6014), TRITC
 
Specifications
Product Data
Clone Name NI 6014 (HP 6014)
Application ELISA, IF, IP, WB
Recommended Dilution To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting using a peroxidase labelled monoclonal antibody against TRITC.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50.
ELISA: from 1/200 upwards.
Western blot: from 1/400 upwards.
Reactivity Human
Antibody Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Highly purified monoclonal IgG2 isolated from pooled Human serum.
Specificity The reactivity of the antiserum is restricted to a subclass specific determinant on the Fab part of the IgG2 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig.
The antiserum does not react with any other component of the human Ig system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
Buffer PBS, pH 7.2 without preservatives or foreign proteins
Label: TRITC
State: Lyophilized purified Ig fraction
Label: Tetramethylrhodamine Isothiocyanate
Absorption emission: 554 nm / 573 nm
Molar radio: Fluorochrome/IgG ~2.7
Reconstitution Method Restore by adding 0.5 ml sterile distilled water
Concentration 0.4 mg/ml
Conjugation TRITC
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
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