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Human IL-22BP ELISA Kit Standard Curve
Human IL-22BP ELISA Kit
RayBio® Human IL-22BP ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 1.64 ng/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 1.64 ng/ml - 400 ng/ml |
| Specificity | Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ALK-1, B7-H2, BLAME, BMP-8, CD28, Common beta Chain, Contactin-1, Desmoglein-1, Desmoglein-3, EDAR, EphA1, EphB6, Ephrin-B3, Epiregulin, FGF-12, FGF-17, FOLR2, Galectin-8, Glypican 1, Glypican 5, GHR, IFNgamma R1, IL-22 R alpha 1, IL-23 R, IL-31 RA, IL-7 R alpha, Integrin alpha 5, MDM2, Nectin-1, NKp30, Nogo Receptor, Notch-3, OSM R beta, Prolactin R, RELT, Ryk, Semaphorin 6D, Semaphorin 7A, Siglec-11. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 3 fold |
| Accession Number | Q969J5 |
| Gene Id | 116379 |
| Gene Symbols | IL22RA2, UNQ5793/PRO19598/PRO19822 |
| Protein Name / Synonyms | Interleukin-22 receptor subunit alpha-2 (IL-22 receptor subunit alpha-2) (IL-22R-alpha-2) (IL-22RA2) (Cytokine receptor class-II member 10) (Cytokine receptor family 2 member 10) (CRF2-10) (Cytokine receptor family type 2, soluble 1) (CRF2-S1) (Interleukin-22-binding protein) (IL-22BP) (IL22BP) (ZcytoR16) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Human IL-22BP qIPCR ELISA Kit
As low as $749.00
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
Customize This Human IL-22BP ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified IL-22BP (IL-22R alpha 2 / IL22RA2), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Proteomic aptamer analysis reveals serum biomarkers associated with disease mechanisms and phenotypes of systemic sclerosis
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Frontiers in immunology
Severe COVID-19 Recovery Is Associated with Timely Acquisition of a Myeloid Cell Immune-Regulatory Phenotype
- Species:
- Human
- Sample Type:
- Serum (severe COVID-19 patients, serial time points)
- Year:
- 2021
- Journal Name:
- Frontiers in Immunology
- PubMed ID:
- 34248984
SARS-CoV2 pneumonia recovery is linked to expansion of innate lymphoid cells type 2 expressing CCR10
- Species:
- Human
- Sample Type:
- Serum (SARS-CoV-2 pneumonia patients)
- Year:
- 2021
- Journal Name:
- European Journal of Immunology
- PubMed ID:
- 34564853
Low Interleukin-22 Binding Protein Is Associated With High Mortality in Alcoholic Hepatitis and Modulates Interleukin-22 Receptor Expression
- Species:
- Human
- Sample Type:
- Plasma (portal vein and peripheral blood)
- Year:
- 2020
