RayBio® C-Series Human Metabolic Pathways Antibody Array 1. Detects Human protein biomarkers from metabolic pathways. Suitable for all liquid sample types.
Human Metabolic Pathways Array C1
Human Metabolic Pathways Array C1
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 41 |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Research Area | Energy Metabolism |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| ADP-Ribosyl Cyclase 2 (BST-1/CD157)
| AHCY
| Alkaline Phosphatase (ALPL)
| Alpha-Galactosidase A (GLA)
| Aminopeptidase N
|
| Arylsulfatase A (ARSA)
| CA9
| CA12
| Carnosine Dipeptidase-1 (CNDP1)
| Catalase
|
| CD38
| CDO
| Chitotriosidase
| Cytochrome C
| Dopa Decarboxylase (DDC)
|
| FOLH1 (PSMA)
| GCNT1
| GLB1 (Beta-galactosidase)
| Glucokinase (GCK)
| GSTP1
|
| HNMT
| Iduronate 2-Sulfatase (IDS)
| Kynureninase
| MIF
| NSE
|
| NUDT5
| PD-ECGF (Thymidine Phosphorylase)
| PLA2G4A
| Sirtuin 1
| Sirtuin 2
|
| Sirtuin 3
| Sirtuin 5
| SMPD1
| SPHK1
| ST3GAL1
|
| ST6GAL1
| ST8SIA1
| SULT2B1
| Thyroid Peroxidase
| TRACP (PAP/ACP5)
|
| Visfatin (PBEF1)
| ||||
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Metabolic Pathways Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.