RayBio® C-Series Human Matrix Metalloproteinase Antibody Array 1 Kit. Detects 10 Human MMPs and TIMPs. Suitable for all liquid sample types.
Human MMP Array C1
Human MMP Array C1
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 10 |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
MMP-1
| MMP-2
| MMP-3
| MMP-8
| MMP-9
|
MMP-10
| MMP-13
| TIMP-1
| TIMP-2
| TIMP-4
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Matrix Metalloproteinase Antibody Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
citations
Viscoelastic hydrogels for interrogating pancreatic cancer-stromal cell interactions
- Year:
- 2023
- Journal Name:
- Materials Today Bio
Loss of the tumor suppressor BTG3 drives a pro-angiogenic tumor microenvironment through HIF-1 activation
- Species:
- Mouse
- Sample Type:
- Conditioned media
- Year:
- 2020
- Journal Name:
- Cell death and disease
- PubMed ID:
- 33311481
Upregulated TIMP1 facilitates and coordinates myometrial contraction by decreasing collagens and cell adhesive capacity during human labor
- Species:
- Human
- Sample Type:
- laboring myometrium
- Year:
- 2023
- Journal Name:
- Molecular Human Reproduction
Effect of Holoptelea integrifolia (Roxb.) Planch. n-Hexane Extract and Its Bioactive Compounds on Wound Healing and Anti-Inflammatory Activity
- Species:
- Human
- Sample Type:
- HaCaT cell
- Year:
- 2022
- Journal Name:
- Molecules
The Secretome of Aged Fibroblasts Promotes EMT-Like Phenotype in Primary Keratinocytes from Elderly Donors through BDNF-TrkB Axis
- Species:
- Human
- Sample Type:
- Conditioned Media
- Year:
- 2020
- Journal Name:
- Journal of Investigative Dermatology
Preseeding of Mesenchymal Stem Cells Increases Integration of an iPSC-Derived CM Sheet into a Cardiac Matrix
- Species:
- Human
- Sample Type:
- Conditioned Media
- Year:
- 2020
- Journal Name:
- ACS Biomaterials Science and Engineering
Effects of osteoblast-derived extracellular vesicles on aggressiveness, redox status and mitochondrial bioenergetics of MNNG/HOS osteosarcoma cells
- Species:
- Human
- Sample Type:
- MNNG/HOS cell
- Year:
- 2022
- Journal Name:
- Frontiers in oncology
Diverse matrix metalloproteinase functionsregulate cancer amoeboid migration
- Species:
- Human
- Sample Type:
- Conditioned Media (A375P and A375M2 cell supernate)
- Year:
- 2014
- Journal Name:
- Nature Communications
Role of ADAM17, p38 MAPK, Cathepsins, and the Proteasome Pathway in the Synthesis and Shedding of Fractalkine/CX3CL1 in Rheumatoid Arthritis.
- Species:
- Human
- Sample Type:
- Conditioned Media (Rheumatoid arthritis synovial fibroblasts)
- Year:
- 2013
- PubMed ID:
- 23897050
EZH2 promotes migration and invasion of triple-negative breast cancer cells via regulating TIMP2-MMP-2/-9 pathway
- Species:
- Human
- Sample Type:
- Conditioned Media (Triple negative breast cancer model with MDA-MB-231 and -468 cells); Conditioned media
- Year:
- 2018
- Journal Name:
- American journal of cancer research