Use code ELISA25-262 for 25% off all ELISAs through November.
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 0.41 ng/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 0.41 ng/ml - 100 ng/ml |
| Specificity | This ELISA antibody pair detects human MMR. Other species not determined. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 30 fold |
| Accession Number | P22897 |
| Gene Id | 4360 |
| Gene Symbols | MRC1, CLEC13D, CLEC13DL, MRC1L1 |
| Protein Name / Synonyms | Macrophage mannose receptor 1 (MMR) (C-type lectin domain family 13 member D) (C-type lectin domain family 13 member D-like) (Macrophage mannose receptor 1-like protein 1) (CD antigen CD206) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
Customize This Human MMR ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified MMR (CD206 / MRC1), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Secretory IgA Is a Key Marker Among Gut Barrier Dysfunction-Related Immunoglobulins Predicting Outcomes in ACLF
- Species:
- Human
- Sample Type:
- Serum (cirrhosis patients, ACLF development)
- Year:
- 2025
- Journal Name:
- Liver International
- PubMed ID:
- 40944472
Radiosensitizing effect of astaxanthin on radiation-induced cytotoxicity in breast cancer cells
- Species:
- Human
- Sample Type:
- Cell Lysate (treated, control cell lysate); Tissue Lysate / Homogenate
- Year:
- 2026
- Journal Name:
- Journal of the Egyptian National Cancer Institute
- PubMed ID:
- 42252369
Cathepsin K regulates the tumor growth and metastasis by IL-17/CTSK/EMT axis and mediates M2 macrophage polarization in castration-resistant prostate cancer
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2022
- Journal Name:
- Cell Death & Disease
Characterizing the lavage and serum cytokine profiles of interstitial pneumonia with autoimmune features and their implications for progressive fibrosis
- Species:
- Human
- Sample Type:
- BALF, serum
- Year:
- 2023
- Journal Name:
- Rheumatology
Bi- and tri-valent T cell engagers deplete tumour-associated macrophages in cancer patient samples
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (malignant ascites supernatant)
- Year:
- 2019
- Journal Name:
- Journal for Immunotherapy of Cancer
- PubMed ID:
- 31753017
Influence of NAFLD and bariatric surgery on hepatic and adipose tissue mitochondrial biogenesis and respiration
- Species:
- Human
- Year:
- 2022
Increased Levels of Soluble CD206 Associated with Rapidly Progressive Interstitial Lung Disease in Patients with Dermatomyositis
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2020
- Journal Name:
- Mediators of Inflammation
Liquid biomarkers of macrophage dysregulation and circulating spike protein illustrate the biological heterogeneity in patients with post-acute sequelae of COVID-19
- Species:
- Human
- Sample Type:
- Plasma
- Year:
- 2022
- Journal Name:
- Journal of Medical Virology
Soluble CD206 levels correlate with disease deterioration and predict prognosis of anti-MDA5 antibody-positive dermatomyositis related interstitial lung disease
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Clinical Respiratory Journal







