Human Ribonuclease 7(RNASE7) ELISA kit
Référence CSB-EL019798HU-96T
Conditionnement : 96T
Marque : Cusabio
Contactez votre distributeur local :
Téléphone : +1 850 650 7790
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
ribonuclease, RNase A family, 7
Associated names
EC 3.1.27.- ELISA Kit; MGC133220 ELISA Kit; Ribonuclease 7 ELISA Kit; ribonuclease, RNase A family, 7 ELISA Kit; RNAS7_HUMAN ELISA Kit; RNase 7 ELISA Kit; RNASE7 ELISA Kit; SAP 2 ELISA Kit; SAP-2 ELISA Kit; Skin derived antimicrobial protein 2 ELISA Kit; Skin-derived antimicrobial protein 2 ELISA Kit
Abbreviation
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, urine, saliva, cell culture supernates, tissue homogenates, cell lysates
Detection Range
1.56 ng/mL-100 ng/mL
Sensitivity
0.39 ng/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Epigenetics and Nuclear Signaling
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of human RNASE7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 100 | ||||
| Range % | 95-105 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 93-103 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 80-95 | |||||
| 1:8 | Average % | 88 | ||||
| Range % | 82-99 | |||||
Recovery
| The recovery of human RNASE7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 95-105 | ||||
| EDTA plasma (n=4) | 104 | 100-109 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Human RNASE7 ELISA Kit was designed for the quantitative measurement of Human RNASE7 protein in serum, plasma, urine, saliva, cell culture supernates, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL .
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Target Background
Function
(From Uniprot)
(From Uniprot)
Exhibits a potent RNase activity. Has broad-spectrum antimicrobial activity against many pathogenic microorganisms and remarkably potent activity (lethal dose of 90% < 30 nM) against a vancomycin resistant Enterococcus faecium. Causes loss of bacterial membrane integrity. Probably contributes to urinary tract sterility. Bactericidal activity is independent of RNase activity.
Gene References into Functions
- These data suggest that the induction of RNase 7 in response to Mycobacterium tuberculosis could have a role in anti-mycobacterial