Human Rubella IgM ELISA Kit

The Human Rubella IgM ELISA Kit is intended for use in the detection of IgM antibody to the rubella virus. Strip well format. Reagents for up to 96 tests.
Purified Rubella antigen is coated on the surface of microwells. Diluted patient serum is added to the wells, and the Rubella IgM- specific antibody, if present, binds to the antigen during incubation. After washing the wells to remove unbound sample, antibody to human IgM conjugated with horseradish peroxidase (HRP) is added and incubated at 37 degree C for 30 minutes. Unbound conjugate is removed by a subsequent washing step. A solution of TMB Reagent is then added to the microwells. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgM-specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls.

Small volumes of IgM elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS8418348 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Rubella IgM, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IgM. The ELISA analytical biochemical technique of the MBS8418348 kit is based on IgM antibody-IgM antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IgM antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IgM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).