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> Human Small proline-rich protein 2B(SPRR2B) ELISA kit

Human Small proline-rich protein 2B(SPRR2B) ELISA kit

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Référence CSB-EL022613HU-96T

Conditionnement : 96T

Marque : Cusabio

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CSB-EL022613HU
Size 96T,5×96T,10×96T

Product Details

Target Name
small proline-rich protein 2B
Associated names
SPRR2B ELISA Kit; Small proline-rich protein 2B ELISA Kit; SPR-2B ELISA Kit
Abbreviation
SPRR2B
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
7.81 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SPRR2B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %90
Range %87-96
1:2Average %103
Range %99-105
1:4Average %93
Range %87-97
1:8Average %91
Range %88-96
Recovery
The recovery of human SPRR2B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9692-101
EDTA plasma (n=4)9086-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.221 2.161 2.191 2.085
10001.864 1.781 1.823 1.717
5001.425 1.546 1.486 1.380
2501.212 1.157 1.185 1.079
1250.746 0.795 0.771 0.665
62.50.357 0.350 0.354 0.248
31.250.236 0.250 0.243 0.137
00.104 0.108 0.106
ELISA Data Analysis
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description

This Human SPRR2B ELISA Kit was designed for the quantitative measurement of Human SPRR2B protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

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Target Background

Function
(From Uniprot)
Cross-linked envelope protein of keratinocytes. It is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. All that results in the formation of an insoluble envelope beneath the plasma membrane.
Gene References into Functions
  1. Knockdown of the Sprr2b gene or inhibition of SPRR2B phosphorylation attenuates USP7/MDM2 binding and p53 degradation, leading to fibroblasts cell cycle arrest.
  2. An SNP in SPRR2B was predictive of asthma among white children with eczema from 2 independent populations.
  3. We showed that just four genes, G3BP2, SCARB2, CSNK1A1 and SPRR2B, can classify patients as presence of lymph node metastasis negative or positive, with 80.0% accuracy.
  4. SPR2B transcripts and protein were moderately increased in the human papillomaviurs 11- and 59-infected tissues. PMID: 14748073.section-table {width: 100%;}.text-default {background: rgb(43, 47, 49);color: white;}.section-table {background-color: blue;}.pdts-relatetitle {padding-left: 5px;line-height: 2;}.pdts-detail-con .item {border-bottom: solid 1.5px rgb(58, 62, 65);display: flex;justify-content: space-between;padding: 9px;}.pdts-detail-con .item:last-child {border-bottom: none;}.pdts-detail-con .item .name {width: 25%;align-self: center;font-weight: 600;font-size: 16px;line-height: 1.5;padding: 5px 0;padding-right: 10px;}.pdts-detail-con .item .value {width: 75%;padding: 5px 0;line-height: 1.5;}table {border-collapse: collapse;}table td {padding: 6px!important;}table tr {border-bottom: solid 1.5px rgb(58, 62, 65);}table tr:last-child {border-bottom: none;}

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