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Human vWF ELISA Kit
Human vWF ELISA Kit
RayBio® Human VWF ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 0.13 ng/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 0.13 ng/ml - 120 ng/ml |
| Specificity | This ELISA antibody pair detects human vWF. Other species not determined. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 8,000 fold |
| Accession Number | P04275 |
| Gene Id | 7450 |
| Gene Symbols | VWF, F8VWF |
| Protein Name / Synonyms | von Willebrand factor (vWF) [Cleaved into: von Willebrand antigen 2 (von Willebrand antigen II)] |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Human vWF qIPCR ELISA Kit
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Target Background
von Willebrand factor (vWF) is a protein synthesized by endothelial cells and megakaryocytes. vWF forms circulating complexes with factor VIII coagulant protein which affects the activation of factor X. It also plays a crucial role in platelet aggregation. Hemophilia A and von Willebrand disease are caused by defects involving factor VIII and vWF. An important hemostatic function of vWF is to promote the adhesion of platelets to the subendothelial matrix.
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human vWF ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified VWF, including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Single-cell Sequencing Reveals Neutrophil Extracellular Traps in Association with Endotheliopathy and Immunothrombosis in Hepatitis B Virus-related Acute-on-chronic Liver Failure
- Species:
- Human
- Sample Type:
- Plasma (HBV-related ACLF patients)
- Year:
- 2026
- Journal Name:
- Journal of clinical and translational hepatology
- PubMed ID:
- 42181839
Role of increased neutrophil extracellular trap formation on acute kidney injury in COVID-19 patients
- Species:
- Human
- Sample Type:
- Plasma
- Year:
- 2023
- Journal Name:
- Frontiers in immunology
Helpful to Live Healthier? Intermittent Hypoxic/Ischemic Training Benefits Vascular Homeostasis and Lipid Metabolism with Activating SIRT1 Pathways in Overweight/Obese Individuals
- Species:
- Human
- Sample Type:
- Serum (overweight/obese individuals)
- Year:
- 2024
- Journal Name:
- Obesity facts
- PubMed ID:
- 38185107
Platelet Transforming Growth Factor-β1 Induces Liver Sinusoidal Endothelial Cells to Secrete Interleukin-6
- Species:
- Human
- Year:
- 2020
- Journal Name:
- Cells
- PubMed ID:
- 32466100
Association of ozone exposure with cardiorespiratory pathophysiologic mechanisms in healthy adults.
- Species:
- Human
- Sample Type:
- Plasma
- Year:
- 2017
- Journal Name:
- JAMA internal medicine
Uremic serum damages endothelium by provoking excessive neutrophil extracellular trap formation
- Species:
- Human
- Sample Type:
- Plasma (uremic patients)
- Year:
- 2021
- Journal Name:
- Scientific Reports
- PubMed ID:
- 34728714
Predictive indicators in peripheral blood and left atrium blood for left atrial spontaneous echo contrast in atrial fibrillation patients
- Species:
- Human
- Sample Type:
- Plasma (plasma peripheral blood; left atrial blood)
- Year:
- 2024
- Journal Name:
- BMC Cardiovascular Disorders
- PubMed ID:
- 39261826
Role of neutrophil extracellular traps in vascular access thrombosis in hemodialysis patients
- Species:
- Human
- Sample Type:
- Plasma
- Year:
- 2023
- Journal Name:
- Thrombosis Research
Shenmayizhi Formula Combined with Ginkgo Extract Tablets for the Treatment of Vascular Dementia: A Randomized, Double-Blind, Controlled Trial
- Species:
- Human
- Sample Type:
- Serum (vascular dementia trial participants)
- Year:
- 2020
- Journal Name:
- Evidence-based Complementary and Alternative Medicine
- PubMed ID:
- 32774430
Vascular endothelial growth factor polymorphism (-460 T/C) is related to hypertension-associated chronic kidney disease
- Species:
- Human
- Sample Type:
- Serum (hypertension associated chronic kidney disease)
- Year:
- 2016
- Journal Name:
- Clinical and Experimental Hypertension
- PubMed ID:
- 27367286