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> IgG2a (HRP)

IgG2a (HRP)

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Référence I1904-80I-HRP-100ul

Conditionnement : 100ul

Marque : US Biological

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I1904-80I-HRP IgG2a (HRP)

Clone Type
Monoclonal
Host
rat
Source
mouse
Isotype
IgG1
Grade
Affinity Purified
Applications
E
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 5ug/ml as coating antibody
Optimal dilutions to be determined by the researcher.

Avidity:
2.5x10e9Me-1

Recommeded Pair:
Coating: I1904-80I
Detection: I1904-83G1

Hybridoma:
Spleen cells from immunized LOU/c rat were fused with cells of the IR983F rat myeloma cell line.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 3H2348 (LO-MG2a-9)|Host: rat|Source: mouse|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).|Purity: Purified by affinity chromatography.|Immunogen: Purified IgG2a from BALB/c mice.|Specificity: Recognizes mouse gamma 2a heavy chain. ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Purified IgG2a from BALB/c mice.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Purity
Purified by affinity chromatography.
Specificity
Recognizes mouse gamma 2a heavy chain.
References
1. Lahat, G. et al. (2010) Vimentin is a novel anti-cancer therapeutic target; insights from in vitro and in vivo mice xenograft studies. PLoS One. 5(4):e10105. 2. Savignac. M. et al. (2010) Increased B cell proliferation and reduced Ig production in DREAM transgenic mice. J Immunol. 185:7527-36 3. Echeverria, P.C. et al. (2006) Potent antigen-specific immunity to Toxoplasma gondii in adjuvant-free vaccination system using Rop2-Leishmania infantum Hsp83 fusion protein. Vaccine. 24: 4102-10. 4. Hajek, A.R. et al. (2008) 12/15-Lipoxygenase deficiency protects mice from allergic airways inflammation and increases secretory IgA levels. J Allergy Clin Immunol. 122: 633-9.e3.