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> IgM (FITC)

IgM (FITC)

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Référence I1905-01A-1mg

Conditionnement : 1mg

Marque : US Biological

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I1905-01AIgM (FITC)

Specifications
Clone TypePolyclonal
Hostgoat
Sourcemouse
IsotypeIgG
GradeAffinity Purified
ApplicationsFLISA IF WB
CrossreactivityMo
Shipping TempBlue Ice
Storage Temp-20°C

Applications:
Suitable for use in FLISA, Western Blot and Immunofluorescence. Other applications not tested.

Recommended Dilution:
FLISA 1:10,000-1:50,000
Immunofluorescence: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.

Absorption Wavelength:
495nm

Emission Wavelength:
528nm

Fluorochrome/Protein Ratio:
3.7 moles FITC per mole of Goat IgG

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. 
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Pab|Isotype: IgG|Host: goat|Source: mouse|Concentration: ~2mg/ml|Form: Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 40% glycerol. Labeled with fluorescein isothiocyanate (FITC).|Purity: Purified by immunoaffinity chromatography and solid phase adsorption to remove any unwanted crossreactivities.|Immunogen: Mouse IgM mu heavy chain.|Specificity: Recognizes mouse IgM mu heavy chain. Assay by IEP resulted in a single precipitin arc against anti-fluorescein, anti-goat serum, mouse IgM and mouse serum. Does not crossreact with mouse IgG or mouse IgA. Exhibits less than 1% crossreactivity with other mouse heavy or light chain isotypes.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Mouse IgM mu heavy chain.
Form
Supplied as a liquid in 0.02M PBS, pH 7.2, 0.15M sodium chloride, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Purity
Purified by immunoaffinity chromatography and solid phase adsorption to remove any unwanted crossreactivities.
Specificity
Recognizes mouse IgM mu heavy chain. Assay by IEP resulted in a single precipitin arc against anti-fluorescein, anti-goat serum, mouse IgM and mouse serum. Does not crossreact with mouse IgG or mouse IgA. Exhibits less than 1% crossreactivity with other mouse heavy or light chain isotypes.