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> IgM (HRP)

IgM (HRP)

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Référence I1905-28-1mg

Conditionnement : 1mg

Marque : US Biological

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I1905-28IgM (HRP)

Specifications
Clone TypePolyclonal
Hostgoat
Sourcemouse
IsotypeIgG
GradeAffinity Purified
ApplicationsE IHC WB
CrossreactivityMo
Shipping TempBlue Ice
Storage Temp-20°C

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:30,000
Western Blot: 1:1000-1:5000
Immunohistochemistry: 1:500-1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Pab|Isotype: IgG|Host: goat|Source: mouse|Concentration: ~1mg/ml (after reconstitution)|Form: Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP). Reconstitute with 1ml sterile ddH2O.|Purity: Purified by immunoaffinity chromatography.|Immunogen: Mouse IgM mu heavy chain|Specificity: Recognizes mouse IgM. Assay by IEP resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, mouse IgM and mouse serum. Specificity was confirmed by ELISA at <1% crossreactivity against other mouse heavy chain isotypes. No reactivity occurs against lambda chain. Some reactivity is observed against kappa chain.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Mouse IgM mu heavy chain
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP). Reconstitute with 1ml sterile ddH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse IgM. Assay by IEP resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, mouse IgM and mouse serum. Specificity was confirmed by ELISA at <1% crossreactivity against other mouse heavy chain isotypes. No reactivity occurs against lambda chain. Some reactivity is observed against kappa chain.