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> IgM (HRP)

IgM (HRP)

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Référence I1905-01Y-500ug

Conditionnement : 500ug

Marque : US Biological

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I1905-01YIgM (HRP)

Specifications
Clone TypePolyclonal
Hostrabbit
Sourcemouse
IsotypeIgG
GradeAffinity Purified
ApplicationsE IHC WB
CrossreactivityMo
Shipping TempBlue Ice
Storage Temp-20°C

Applications:
Suitable for Western Blot, ELISA and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:1000-1:5000
ELISA: 1:180,000
Immunohistochemistry: 1:500-1:2500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Pab|Isotype: IgG|Host: rabbit|Source: mouse|Concentration: ~1.5mg/ml|Form: Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Labeled with Horseradish Peroxidase (HRP).|Purity: Purified by immunoaffinity chromatography.|Immunogen: Mouse IgM whole molecule |Specificity: Recognizes mouse IgM. Assay by IEP resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum, mouse IgM and mouse serum. No reaction was observed against other mouse heavy and light chain proteins||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Mouse IgM whole molecule
Form
Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Labeled with Horseradish Peroxidase (HRP).
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse IgM. Assay by IEP resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum, mouse IgM and mouse serum. No reaction was observed against other mouse heavy and light chain proteins