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> Infectious Peritonitis, Feline (FIPV) (APC)

Infectious Peritonitis, Feline (FIPV) (APC)

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Référence I7600-51-APC-100ul

Conditionnement : 100ul

Marque : US Biological

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I7600-51-APC Infectious Peritonitis, Feline (FIPV) (APC)

Clone Type
Monoclonal
Host
mouse
Isotype
IgG2a
Grade
Affinity Purified
Applications
FC FLISA IF IHC WB
Crossreactivity
Fe
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry, FLISA, Western Blot and Immunofluorescence. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (Frozen, paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin embedded sections. Sodium citrate buffer, pH 6.0 is recommended.
Western Blot (Reducing conditions): Detects a band of 50-56kD that represents the nucleocapsid. Also detects specific bands at 56 and 46kD in reducing gels with canine coronavirus (CCV) as the antigen.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. 

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG2a|Clone No: 3H2460 (FIPV3-70)|Host: mouse|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).|Purity: Purified by Protein A affinity chromatography from tissue culture supernatant.|Immunogen: Coronavirus cocktail|Specificity: Recognizes feline infectious peritonitis virus (FIPV) type 1 and 2. Specific for the nucleocapsid. Shown to react with canine coronavirus (CCV), porcine coronavirus transmissable gastroenteritis virus (TGEV) and ferret coronavirus. Some reactivity has been observed against bovine coronavirus (BCV). Exhibits negative reactivity against feline leukemia virus, feline immunodeficiency virus, feline calcivirus, feline Herpes virus, canine adenovirus (type 2), canine distemper virus, canine parvovirus and canine parainfluenza virus.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Coronavirus cocktail
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein A affinity chromatography from tissue culture supernatant.
Specificity
Recognizes feline infectious peritonitis virus (FIPV) type 1 and 2. Specific for the nucleocapsid. Shown to react with canine coronavirus (CCV), porcine coronavirus transmissable gastroenteritis virus (TGEV) and ferret coronavirus. Some reactivity has been observed against bovine coronavirus (BCV). Exhibits negative reactivity against feline leukemia virus, feline immunodeficiency virus, feline calcivirus, feline Herpes virus, canine adenovirus (type 2), canine distemper virus, canine parvovirus and canine parainfluenza virus.
References
1. Kipar, A., et al., J. Comp. Path. 119: 1-14 (1998). 2. Kipar, A., et al., Vet. Pathol. 37: 129-136 (2000). 3. Michimae, Y. et al. (2010) J. Toxicol. Pathol. 23: 99-101. 4. Suderman, M.T. et al. (2006) NASA/TP-2006-213723.