ELISA. Western Blot: This antibody cross reacts with IFN beta in western blots but does not show any cross reactivity with IFN gamma; this antibdoy detects a band of approximately 19kDa. Functional Assays. Immunoprecipitation.
Reactivity
Chicken
Antibody Host
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
Recombinant chicken Interferon alpha.
Specificity
This antibody recognizes Interferon alpha (chIFN-alpha). In bioassays, this antiserum neutralizes chIFN-alpha, but not chIFN-beta, and can therefore be used to discriminate IFN alpha and IFN beta bioactivities. Product SA027 is the immunogen used to produce this antibody.
Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Stability
Shelf life: one year from despatch.
Shipping
Blue Ice
Background
The classification of mammalian cytokines with antiviral activity, is well documented as being either Type I Interferon (IFNI) or Type II Interferon (IFNII). This is not the case for avian IFNs however, even though IFN was first detected in the chicken. A growing number of functional studies indicate similarities between chicken IFN and mammalian IFNI, revealing the conservation of cysteine residues and the existence of around ten closely related intron-less IFN alpha genes. Further research has shown that chIFN-alpha genes are strongly expressed following viral infection of monocyte-derived macrophage and embryo fibroblasts and that recombinant chicken IFN acts as a potent antiviral agent.
Synonyms
B Cell Interferon, IFN-alpha, IFNA, Interferon-alpha, Leukocyte Interferon, Type I Interferon
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