LSD1, Homogeneous, BioAssay™ Kit (Lysine-Specific Histone Demethylase, KDM1A, AOF2, BHC110)

LSD1 is a chromatin-modifying enzyme that specifically removes methyl groups from mono- and dimethylated Lys of histone H3. LSD1 is a critical component of transcriptional regulation via epigenetic histone modifications and is therefore a potential target for drug development.

Intended Use:
The LSD1 Homogeneous BioAssay™ Kit is designed to measure activity of lysine-specific demethylase (LSD1) for screening and profiling applications.

Test Principle:
The key to the LSD1 Homogeneous BioAssay™ Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for demethylase detection. First, a sample containing LSD1 enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

Applications:
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Kit Components:
LSD1, 1x10ug
Primary antibody 10, 1x40ul
Biotinylated histone H3 peptide substrate, 1x400ul
3x LSD1 assay buffer 2, 1x3ml
4x Detection buffer, 1x2ml

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.